FastQCFastQC Report
Thu 26 May 2016
SRR1511888_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511888_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1268345
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT38000.2996030259905625No Hit
TATCAACGCAGAGTACTTTTTTTTT23720.18701536253937218No Hit
GGTATCAACGCAGAGTACTTTTTTT22510.17747537144862005No Hit
CTGTAGGACGTGGAATATGGCAAGA15720.12394104127820113No Hit
GTCCTAAAGTGTGTATTTCTCATTT14790.11660865143158998No Hit
GTCCTACAGTGGACATTTCTAAATT14420.11369146407326082No Hit
CTTTAGGACGTGAAATATGGCGAGG14020.11053774801020226No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7650.014.3549941
CTTATAC759.618961E-713.93973
CTACACG550.003057427512.0964354
ATCCTAC1156.579103E-811.6251021
TGCGCCA855.358201E-511.16879512
TGTGCCG600.005904763511.07572210
GACTCTA600.005904763511.0757227
GTCTTAG1408.972711E-810.23127651
TAGGACC2053.6379788E-1210.199784
ACGTTTT750.002659514810.12757117
GGCGAGG4050.010.0866519
GTATAAG1803.583409E-1010.0797011
TAGAGGT1701.544322E-910.063424
ACTGACC856.632922E-410.0519158
GCGCCAC951.6527386E-49.99431313
TAGGGTT1554.0286977E-89.8109335
TAAGACT1651.0128133E-89.7923524
GTCCTAA5200.09.7328291
GTGGTAT2303.6379788E-129.54919051
TCTACAC1901.0404619E-99.5043413
Produced by FastQC (version 0.11.5)