FastQCFastQC Report
Thu 26 May 2016
SRR1511887_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511887_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences596118
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT13820.2318332947503682No Hit
TATCAACGCAGAGTACTTTTTTTTT8010.13436936982275322No Hit
GGTATCAACGCAGAGTACTTTTTTT8010.13436936982275322No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG307.721976E-418.9974845
TCGAACT350.002170008616.28355619
GTAATAC500.001499152513.2982393
GGTATCA2850.013.0146531
TGTAATA751.4579751E-512.68094352
GATGTAG550.003038876712.1045381
CCTAGGT550.003067673412.0893093
CTATAGT550.003067673412.0893094
GAGCGTG550.003067673412.0893099
ACTGTGC1353.6925485E-1011.9613798
CTACACT658.019605E-411.690764
AAGGCTA658.019605E-411.690765
GTTTAGG752.0481794E-411.4128491
CTTATAC752.0722776E-411.398493
GTATCAA7150.011.3064371
TCCTAGG855.2492695E-511.1890692
ATTATAC600.005874250511.0818653
CTGTGCG600.005874250511.0818659
CCAGTAC951.3581826E-510.9985443
GTTCTAA700.00147513410.869381
Produced by FastQC (version 0.11.5)