FastQCFastQC Report
Thu 26 May 2016
SRR1511887_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511887_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences596118
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT15730.2638739310002382No Hit
TATCAACGCAGAGTACTTTTTTTTT9830.16490023787236754No Hit
GGTATCAACGCAGAGTACTTTTTTT9070.1521510841813198No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTACG250.005871841719.086411
GTCCATA653.2089156E-614.6818541
TAGACAC655.424594E-513.1582055
GGTATCA2850.013.0591231
GTATAAA853.7372156E-612.350031
GTTCTAG550.00296219112.1458981
GTATCAA7650.012.10053351
CCTAGAC550.003057031712.0949163
ATAAATC550.003057031712.0949163
TCTACAC951.0320528E-612.0039773
TTAGACA657.988446E-411.6961824
CACCGTG658.044894E-411.6863727
GTGGTAT1001.820621E-611.4518461
TGTAATA752.0633647E-411.4037772
TAAAGCT600.00585399611.0870074
TAAGACT700.001484954710.860744
TATAAAT803.7518184E-410.6910412
GTCTAGG750.002545774710.17941951
CTAGACT750.00263621810.1366914
TCTAGGA750.00263621810.1366912
Produced by FastQC (version 0.11.5)