FastQCFastQC Report
Thu 26 May 2016
SRR1511886_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511886_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1293466
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT29900.23116185504682765No Hit
TATCAACGCAGAGTACTTTTTTTTT17960.1388517363425092No Hit
GGTATCAACGCAGAGTACTTTTTTT16870.13042476570702285No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6150.012.0624761
CCCTTAT951.34258025E-511.0125041
GTATCAA14700.010.8047851
CTAGCAC1605.4387783E-1010.6859993
TATAAGG1451.41626515E-810.4946752
GTCCTAA4000.010.4618791
AATCCCG1002.4002125E-510.44893719
GTATAAT1855.820766E-1110.2819451
TAATACT2053.6379788E-1210.193694
TCGAACT951.6450271E-49.99898219
TTATAGT1151.0274252E-59.9116524
TAAGACC1151.0274252E-59.9116524
TGTTAGG1451.5886326E-79.83875752
GGACGTG4350.09.8262066
AGGACGT4850.09.7924395
GTGCTAG1752.5193003E-99.7825361
GGCTTAA900.00110173769.5107991
GTCTTAA1701.6314516E-89.5107981
CTCGAAC1106.839898E-59.49903418
AAATCCC1606.635855E-89.49903318
Produced by FastQC (version 0.11.5)