FastQCFastQC Report
Thu 26 May 2016
SRR1511886_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511886_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1293466
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT30970.23943420236790142No Hit
GGTATCAACGCAGAGTACTTTTTTT19500.1507577315522789No Hit
TATCAACGCAGAGTACTTTTTTTTT19100.14766526526402704No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6500.014.3926431
ACCGATC400.00530161114.2406068
GCGTGCG802.013514E-613.0538899
GTTCTAG959.802225E-712.0582831
CGTGCGC802.8860317E-511.86671410
AGTCGTC658.06632E-411.68468
AACTTGC752.0860389E-411.3924857
GTATCAA15600.011.075971
AAGGCGT951.3596238E-510.9999786
GCGCCAC951.3695675E-510.99274813
TAGGACC2001.8189894E-1210.4568554
TACCCTC1105.9944086E-610.3704365
TATTAGG1303.8033068E-710.2374812
GTCCTAC4900.010.1305981
CCCCTAA856.3041045E-410.1076781
GTATAAG1803.601599E-1010.0764811
TACACGG856.551718E-410.0654225
GTTGAGT856.6314253E-410.0521939
CTACTAA1053.889985E-510.0007181
AGGCGTG951.6543089E-49.9934077
Produced by FastQC (version 0.11.5)