FastQCFastQC Report
Thu 26 May 2016
SRR1511885_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511885_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1369027
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT28280.20657006764658406No Hit
GGTATCAACGCAGAGTACTTTTTTT17910.13082283987094484No Hit
TATCAACGCAGAGTACTTTTTTTTT17380.12695147721703076No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7300.013.6782421
CTACACG600.00588026611.0820134
GTCTACG700.001478647110.8681551
GGACCGC700.001492689410.8558486
AGGACGT4350.010.6998735
GTAAGGG1252.2421955E-710.6387333
TTAGGAC2350.010.5093863
GGACGTG4400.010.3624016
GACGTGG2450.010.0804327
GTCCTAT1451.5918158E-79.8375541
TATGCTG2054.1836756E-119.7305485
GTATCAA14950.09.6050491
GTCCTAG1401.0431013E-69.5096351
GTCCTAC3600.09.5096351
TCTACGA800.00447727049.5096352
TATTAGG1304.181067E-69.5096352
GTGGTAT2600.09.5096351
TATAAGA1606.524351E-89.5096352
TTTAGAC1201.7017654E-59.4988683
TAGGACG5000.09.4988684
Produced by FastQC (version 0.11.5)