FastQCFastQC Report
Thu 26 May 2016
SRR1511885_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511885_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1369027
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT33820.2470367640667423No Hit
TATCAACGCAGAGTACTTTTTTTTT22210.16223200857251172No Hit
GGTATCAACGCAGAGTACTTTTTTT20190.14747700374061284No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGCCC250.006054605418.98754510
TAAGACG350.002163115716.2934884
GGTATCA7950.012.8510031
AGGACGT4550.012.1156695
GGACGTG4800.012.0720586
TAGGACG4750.012.0057274
GTATAGG751.9782878E-411.4578091
GCGCCAC1001.9424006E-611.3933613
GACGTGG2950.011.264217
GTAGGAC4500.011.1942293
CGTGGAA3250.011.1008179
CCTATAC1053.4448658E-610.8623253
GGCGTGC803.7916852E-410.6808858
CGTGCGC803.792965E-410.68049510
GTATTAA2001.8189894E-1210.5029921
ACGTGGA3300.010.3572228
AAAGGCG1201.5134283E-610.2965795
GTGGTAT2600.010.2826491
TGTAGGA5000.010.2648962
GTATAAT2150.010.2143271
Produced by FastQC (version 0.11.5)