FastQCFastQC Report
Thu 26 May 2016
SRR1511884_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511884_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1349125
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT24140.17893078847401092No Hit
GGTATCAACGCAGAGTACTTTTTTT14230.10547577133327157No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6350.012.5786091
AAGGCGT701.0918073E-412.2129536
GTCTTAT803.7377514E-410.6974331
ATTATAC1701.382432E-1010.61648753
CTTACAC2100.010.4036253
GTGGTAT2400.010.3012321
TACACCG750.002649583610.1322275
TTACACA2650.010.0366394
TAGGATA1451.6179911E-79.8265124
GTATCAA12700.09.808321
TGGTATC2303.6379788E-129.508832
TAATAGT1201.7015076E-59.4989624
TTACCCT1201.7015076E-59.4989624
GTATGAG1752.6255293E-89.2371491
GTGCTAG1554.198555E-79.2020931
GTCCTAC3750.09.1284761
AGACTAT1151.11131776E-49.0859646
TTAGTAC1151.11131776E-49.0859643
TAGGACG2950.09.01596454
TCTTATA1804.148933E-88.9805612
Produced by FastQC (version 0.11.5)