FastQCFastQC Report
Thu 26 May 2016
SRR1511884_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511884_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1349125
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT27800.2060594829982396No Hit
GGTATCAACGCAGAGTACTTTTTTT16260.12052256091911424No Hit
TATCAACGCAGAGTACTTTTTTTTT16020.11874363013064024No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6450.014.3486551
TATACCG604.0796268E-412.6709955
GTATTAT1751.8189894E-1110.9041331
GTATAAA2800.010.5633771
GCGCCAC1106.0764232E-610.35872413
CCTATTA750.002554001510.177191
GTATCAA14100.010.0824551
GGTCTAC1553.796049E-89.8488931
GTGGTAT2002.4374458E-109.5411161
CTAAGGC1201.6924485E-59.5032463
ATTATAC1401.052651E-69.5032453
TGGCGAG2800.09.50007318
GTCTAGA2451.8189894E-129.3463991
GTATAGA2252.3646862E-119.329091
GTATTAA1659.9582394E-89.2519911
GTATAAC1451.6074464E-69.2121111
CCATTAT1252.615492E-59.15947151
GTGTAGA2409.094947E-129.143571
CGCCACC1054.5118073E-49.04332914
ACTAGAC950.00181747419.0030753
Produced by FastQC (version 0.11.5)