FastQCFastQC Report
Thu 26 May 2016
SRR1511883_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511883_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1191275
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT27270.2289143984386477No Hit
GGTATCAACGCAGAGTACTTTTTTT16660.13985016054227614No Hit
TATCAACGCAGAGTACTTTTTTTTT14860.12474029925919708No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6350.011.0816881
GTGGTAT3100.011.0430421
ACACCGT951.36107665E-510.9987366
CCTAGAC1408.352799E-910.8558953
CTAGTTA700.001492389410.85589513
TGGTATC2900.010.8209112
TTAGACT1158.8211164E-710.7378974
GTACTAG803.7354016E-410.6979471
AGGACCG909.520956E-510.5543435
ACTGTTC2850.010.3321468
GTCCTAA1606.0626917E-910.1036161
TACACTG5000.010.0688445
ATAGACC1054.107203E-59.9512393
GTCCATA1151.0136535E-59.9227331
CTTAGAC1151.0268952E-59.9119053
ATAACTG1451.6173544E-79.8264575
GTTCAGG1856.348273E-109.7662941
GTAGGAC2151.0913936E-119.7198133
GTCCTAC2201.6370905E-119.5092861
TACTAGA900.00110320719.5092862
Produced by FastQC (version 0.11.5)