FastQCFastQC Report
Thu 26 May 2016
SRR1511883_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511883_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1191275
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT32680.2743279259616797No Hit
GGTATCAACGCAGAGTACTTTTTTT20540.172420305974691No Hit
TATCAACGCAGAGTACTTTTTTTTT17330.14547438668653334No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCATGCG402.7734035E-416.614799
CGTGCGC508.7601155E-515.19002710
ACAGCGG701.0967589E-412.2067848
GGTATCA6150.011.4850991
CTCGGAC600.00585344511.0886283
CATCCCG600.005903060511.07606110
GGTATAT1053.2724838E-610.9086261
TAGTACC700.001485028710.86232954
AGGTTGC700.001492333110.8559446
GTATCAA13200.010.3404691
TAGGACC1303.8115286E-710.2356574
GACTGTC1606.2227627E-910.0875517
GTGGTAT2650.010.0853341
GTCTAGT1451.5070327E-79.8741871
GTCTTAG1659.440555E-99.8342921
GTCCTAC2053.8198777E-119.7778541
GTCTAGG1201.6062368E-59.5450481
TAGAGGT1401.0502608E-69.5045394
TGGACAG3200.09.5045395
TAGACTG1304.2080046E-69.5045385
Produced by FastQC (version 0.11.5)