FastQCFastQC Report
Thu 26 May 2016
SRR1511882_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511882_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1562049
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT28830.18456527292037575No Hit
TATCAACGCAGAGTACTTTTTTTTT16870.10799917288126044No Hit
GGTATCAACGCAGAGTACTTTTTTT15970.10223750983483872No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCCCG551.9623482E-413.8163655
GGTATCA7950.012.6808341
CCGCGTG550.003071479312.089329
GTATAAG1552.5465852E-1111.6581851
TAGACTG1701.0913936E-1111.1750025
GGCGAGG2300.011.15070919
TATGACG600.00588143311.0818774
TACCGTA600.00588143311.0818777
AGGACGT5500.010.8803885
GGACGTG5450.010.805926
GACGTGA2950.010.6257237
GTGTAGG2700.010.567361
TAGGACG5900.010.4647264
TAGGACT2001.8189894E-1210.4486274
GATCTAC1502.401248E-810.1446671
CGTGAAA3200.010.0924249
GTACTAG951.6253957E-410.0111841
GCCGGTT951.6460159E-49.99868711
GTAATAC1151.027753E-59.9117413
TTAGGAC3750.09.8787013
Produced by FastQC (version 0.11.5)