FastQCFastQC Report
Thu 26 May 2016
SRR1511882_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511882_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1562049
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT32720.2094684609765763No Hit
GGTATCAACGCAGAGTACTTTTTTT19410.12425986636782842No Hit
TATCAACGCAGAGTACTTTTTTTTT19310.12361968158489266No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAC350.002164933116.2916473
GGTATCA7550.013.3971191
GTGGTAT2850.011.3837811
CTTATAC1451.209628E-911.1419883
GACTGCG600.005899014411.0774347
TGGCGAG4650.011.03203518
GTATTAG1053.2860007E-610.9054711
TAGGACT1752.0008883E-1110.8610974
CCAACGA700.001491262310.8572719
GGCGAGG1951.8189894E-1210.71807619
CTGTCCG803.7892957E-410.681819
GTATAAG2001.8189894E-1210.4965151
GTATCAA16650.010.3732981
GATATAC3350.010.2543981
ATGGCGA5400.010.19859717
TATGGCG5150.010.14055416
TAATACT2450.010.0853044
TAATACC1701.6534614E-89.503464
ATAAGGC1106.807556E-59.503463
CTTACTC1502.6326234E-79.503463
Produced by FastQC (version 0.11.5)