FastQCFastQC Report
Thu 26 May 2016
SRR1511881_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511881_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1179908
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT20750.17586116883689237No Hit
GTCCTACAGTGGACATTTCTAAATT14770.12517925126365784No Hit
CTGTAGGACGTGGAATATGGCAAGA13950.11822955688070595No Hit
GGTATCAACGCAGAGTACTTTTTTT12260.10390640626218314No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG655.4555672E-513.15249618
GGCGAGG2300.012.80303419
GGTATCA5650.012.7899151
CCAACGA604.0942695E-412.66536819
TACACCG604.0942695E-412.6653685
CTGTGCG550.003070051312.0896689
TAGGACC2950.011.9140324
ATAGGAC1950.011.6911093
GTATTAA1156.9809175E-811.5753141
CAGGACG752.0749847E-411.398834
GTCCTAA3750.011.1563971
AGGACGG951.3608613E-510.9988715
TAGGACT2600.010.5950664
GTGTAGG1909.094947E-1210.5091661
TAAGACA1553.6616257E-910.4182864
GTAGGAC7400.010.3975813
TGTAGGA7550.010.3268882
GTCTTAG1201.5051064E-610.3006521
TTATACA1951.4551915E-1110.229724
CTAAGAC1409.688483E-810.1775263
Produced by FastQC (version 0.11.5)