FastQCFastQC Report
Thu 26 May 2016
SRR1511881_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511881_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1179908
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT23510.19925282310146214No Hit
GTCCTACAGTGGACATTTCTAAATT16110.1365360689138475No Hit
CTGTAGGACGTGGAATATGGCAAGA14380.1218739088132295No Hit
GGTATCAACGCAGAGTACTTTTTTT13550.1148394620597538No Hit
TATCAACGCAGAGTACTTTTTTTTT13280.11255114805561112No Hit
GTCCTAAAGTGTGTATTTCTCATTT12140.1028893778158975No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTTAC905.116726E-712.7233061
GGACCGT550.003074516512.0873226
GTATCAA11100.011.8636241
TCCCGTT658.05234E-411.68685711
TAGGACA2200.011.6620584
GTCCTAA4500.011.238921
CTTATGC600.005863121211.0861533
GTGTAGC2250.011.0268651
TGTGCGG700.001496751310.85208210
GGTATCA4750.010.8482931
TGTACTG2550.010.806675
CTGGTCG909.554755E-510.5506359
TGTTACA1106.0217444E-610.3662742
GTAGGAC6450.010.3127013
CTAGACA1201.5170917E-610.2942864
TAGGACC2053.6379788E-1210.1977164
AAAGTGT4850.010.1825466
CTATTAG750.002550565610.1786451
GACAGTC1502.4581823E-810.1286117
GTATAAT1909.094947E-1110.0447151
Produced by FastQC (version 0.11.5)