FastQCFastQC Report
Thu 26 May 2016
SRR1511880_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511880_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1428755
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT21580.15104059128402No Hit
GTCCTACAGTGGACATTTCTAAATT20990.14691112192083317No Hit
CTGTAGGACGTGGAATATGGCAAGA20540.14376152664382627No Hit
GTCCTAAAGTGTGTATTTCTCATTT20180.14124185042222076No Hit
CTTTAGGACGTGAAATATGGCGAGG19470.1362724889851654No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCGC400.005283344514.2481628
CTCCTCG400.005283344514.24816218
AAGTCGT907.4699874E-611.6096137
GTATCAA12450.011.3055641
TAGGACC3400.011.1750294
ACCACGC600.00588090711.08190418
AAAGCGG600.00588090711.0819045
CGAAATC1053.4703262E-610.85574313
ACGAAAT1053.4703262E-610.85574312
GGTATCA5550.010.7956161
GGACGTG11050.010.745226
ATAAGAC1252.2427957E-710.6386293
TATACTG2950.010.6257495
TGTTATA909.407731E-510.5671382
GACTTAT1002.369151E-510.4614661
GACGTGG5950.010.3768137
AGGACGT11700.010.1482655
CAAGTCG951.6457522E-49.9987116
ACGTGGA6400.09.7956118
TAATACT1951.6370905E-109.7423334
Produced by FastQC (version 0.11.5)