FastQCFastQC Report
Thu 26 May 2016
SRR1511880_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511880_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1428755
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT24560.1718979111184213No Hit
GTCCTACAGTGGACATTTCTAAATT22840.15985945805963933No Hit
CTGTAGGACGTGGAATATGGCAAGA21920.1534202854933141No Hit
GTCCTAAAGTGTGTATTTCTCATTT21910.1533502944871584No Hit
CTTTAGGACGTGAAATATGGCGAGG20750.14523133777309616No Hit
TATCAACGCAGAGTACTTTTTTTTT15480.10834607752903752No Hit
GGTATCAACGCAGAGTACTTTTTTT14290.10001714779650815No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTGCG402.7723136E-416.6160289
CGTGCGC402.7730537E-416.61544610
GACAGTC1650.013.8107247
GGTATCA6050.012.9374531
TAGGACC3050.012.4636344
TCCTATA2100.011.7662632
GTATTAG1354.3855835E-911.3129661
GGCGTGC855.3512736E-511.1704398
GTATCAA14050.010.8021721
GGCGAGG4750.010.79832819
AATCCCG909.527861E-510.55392119
AAGGCGT1002.4030263E-510.4480166
GGACGTG10600.010.3942546
AGGACGT11250.010.2215645
GTATTAT1701.4333636E-910.1068041
AATGTCC4550.09.8078918
TCTTATA2450.09.6974692
TGTCCAC4900.09.68830710
GACGTGA6650.09.5662627
GGCCTAT1201.6064205E-59.5453161
Produced by FastQC (version 0.11.5)