FastQCFastQC Report
Thu 26 May 2016
SRR1511879_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511879_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1094239
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT21580.1972146852744236No Hit
GGTATCAACGCAGAGTACTTTTTTT12640.11551406959539917No Hit
TATCAACGCAGAGTACTTTTTTTTT11420.10436476857432425No Hit
GTCCTACAGTGGACATTTCTAAATT11260.10290256516172426No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCAACG350.00215605216.3010462
ACCGTGC604.094126E-412.6652658
CTAATAG701.09145774E-412.2129353
ATTATAC1052.7207352E-711.7606043
CTAGGAC1552.5465852E-1111.6438723
TCCCGTT600.00587861511.08210711
TAAGGTC600.00587861511.0821075
GTCCTAC4400.010.8056171
TAGGACC2050.010.6573574
GGTATCA4300.010.6146341
GTGGTAT1909.094947E-1210.5098851
GTATAGG1105.971331E-610.3733921
GTATCAA9500.010.3096961
GTCCTAG1303.7861537E-710.24041
TTAGACC856.596328E-410.0577114
GTAGACC2650.010.0366263
TATTAGG951.6272864E-410.0094142
GGACGTG5250.09.9512816
GGTCTAC1151.0138459E-59.9223761
GTCTTAG1356.3500374E-79.8611261
Produced by FastQC (version 0.11.5)