FastQCFastQC Report
Thu 26 May 2016
SRR1511879_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511879_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1094239
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT24710.2258190395334109No Hit
GGTATCAACGCAGAGTACTTTTTTT14140.1292222265885241No Hit
TATCAACGCAGAGTACTTTTTTTTT12660.11569684502197417No Hit
GTCCTACAGTGGACATTTCTAAATT12120.11076190850444921No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGTGC502.1893902E-718.9854248
AAGGCGT704.427402E-714.930086
GCGTGCG456.799514E-414.766449
GGTATCA6350.013.0864911
GTATAAT1501.8189894E-1212.7355131
AAAGGCG853.909754E-612.3026765
TGTAGGA6350.011.9768342
GAGTAGC802.7099157E-511.9395441
AGGACCG657.9734024E-411.7004455
GTAGGAC5800.011.6374063
TCCAACG907.4577856E-611.61069218
TCGAACT907.4577856E-611.61069219
CTTATAC1001.911596E-611.4079363
CTAGACC855.2817057E-511.1842513
CCTACGT600.00584342411.0910482
TACGTCT600.00584342411.0910484
AGGCGTG951.37067345E-510.9915627
GCCTCGA1158.8878005E-710.73138116
CTGTAGG6600.010.7094091
GCGCCAC803.7939695E-410.67978913
Produced by FastQC (version 0.11.5)