FastQCFastQC Report
Thu 26 May 2016
SRR1511878_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511878_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences895389
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT16940.18919151340925563No Hit
TATCAACGCAGAGTACTTTTTTTTT10600.11838430000815287No Hit
GGTATCAACGCAGAGTACTTTTTTT10560.11793756680057496No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGC350.002171193216.28351410
GGCGTGC602.5691354E-514.2480748
AGGCGTG602.5691354E-514.2480747
GGACCGT602.5691354E-514.2480746
AAGGCGT551.9609391E-413.8163156
GGTATCA4250.013.6509831
ACCGTGA500.001500209213.29820258
TCTAGAC957.3860065E-812.9982423
TAACCAC604.093682E-412.6649545
ATAAGAC751.47898045E-512.6649543
TATTCTA550.00306980612.0892755
CTAGACT855.3241125E-511.1749594
CTTAGAC855.3241125E-511.1749593
CTAGACC700.001492014510.8556764
TAGACTG1408.341885E-910.8556765
ATTGTAC700.001492014510.8556763
GCGCCAC700.001492014510.85567613
CTCTAGG909.393628E-510.5677021
GTAGACT909.516858E-510.5541294
AACAGAT1451.441731E-810.4813417
Produced by FastQC (version 0.11.5)