FastQCFastQC Report
Thu 26 May 2016
SRR1511878_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511878_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences895389
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT19600.21889927171318838No Hit
GGTATCAACGCAGAGTACTTTTTTT12990.1450766091609345No Hit
TATCAACGCAGAGTACTTTTTTTTT11970.13368491236769717No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAC1300.013.9466751
GTCTTAG1300.013.9466751
GTCTAGA706.9276193E-613.6320891
GGTATCA5200.013.212641
TCAATAC751.4704598E-512.67207053
TAAACAG1800.012.1440685
AGAGGCG657.988943E-411.6972965
CTGTGCG951.3670471E-510.993639
GGTTATA700.001435886310.9056711
GTGGTAT1751.8189894E-1110.905671
CTAGAAC1053.4426066E-610.8617753
CATAAGA803.7524832E-410.69205952
GTATCAA10450.010.5925891
GTCTAAG1002.2801773E-510.4967081
GACAATG1201.531851E-610.2857077
ACAGGTC1451.6261038E-79.8218968
TCTTAGA1752.5429472E-99.7755982
ACTGTCC1752.588422E-99.7657718
TCCTACA1856.4028427E-109.760922
GTACAAG1303.995756E-69.5424621
Produced by FastQC (version 0.11.5)