FastQCFastQC Report
Thu 26 May 2016
SRR1511877_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511877_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences923798
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT24020.2600135527463796No Hit
TATCAACGCAGAGTACTTTTTTTTT14520.15717721839622947No Hit
GGTATCAACGCAGAGTACTTTTTTT13900.15046579447021968No Hit
GTCCTAAAGTGTGTATTTCTCATTT10740.11625918220216974No Hit
GTCCTACAGTGGACATTTCTAAATT10470.11333646533116548No Hit
CTGTAGGACGTGGAATATGGCAAGA10290.11138798741716263No Hit
CTTTAGGACGTGAAATATGGCGAGG9520.10305283189615046No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGTCGA508.716311E-515.19826817
TAAGACT759.672913E-713.9317464
CCAACGA551.9606912E-413.81660819
GGTATCA4450.013.4624791
CTAGGTT852.6987436E-713.4102364
TAGGACC2150.013.2543034
TCCAACG957.384915E-812.99851918
GACGTGA3650.012.23148257
TGGCGAG6400.012.17048718
GGACTAG550.003044640912.1026341
GGCGAGG3250.011.9832519
GTTTAGG1209.84619E-911.8865151
GTAGGAC6800.011.7339573
CTTAAGC658.025684E-411.6909753
GTGGTAT1001.9042636E-611.4110541
TATGGCG7000.011.39870116
ATGGCGA7050.011.31785917
GGACGTG6450.011.1925236
AGGACGT6750.011.1172515
GGGTAGG600.00583038311.094081
Produced by FastQC (version 0.11.5)