FastQCFastQC Report
Thu 26 May 2016
SRR1511877_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511877_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences923798
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT32400.35072602452051205No Hit
TATCAACGCAGAGTACTTTTTTTTT21430.23197712053933867No Hit
GGTATCAACGCAGAGTACTTTTTTT19880.2151985607243142No Hit
CTGTAGGACGTGGAATATGGCAAGA10740.11625918220216974No Hit
ACGCAGAGTACTTTTTTTTTTTTTT10620.11496019692616784No Hit
CTTTAGGACGTGAAATATGGCGAGG10050.10879001686515882No Hit
GTACTTTTTTTTTTTTTTTTTTTTT9840.10651679263215552No Hit
GTCCTACAGTGGACATTTCTAAATT9600.10391882208015171No Hit
GTCCTAAAGTGTGTATTTCTCATTT9580.1037023245341514No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG1450.014.4211115
CCATTAG400.005133806314.3091821
GGTATCA5600.012.7760551
ATAGGAC604.07176E-412.6730963
ACTGCCC1052.7354145E-711.75513658
ACATTCG658.01667E-411.69254118
CTAAACT752.0623318E-411.4057874
TCGAACT752.0716935E-411.40022719
GGCGAGG2700.011.25948419
GTATACT600.00585045411.0889594
TGGCGAG5500.011.05476618
CTCTTAG1407.8052835E-910.9022341
TAAGCTG700.001484111810.8626555
GACGTGA3000.010.760477
CAATACT803.749921E-410.6929254
GTATCAA12400.010.616491
ATTTAGA2350.010.5542911
TAGGACC1909.094947E-1210.505334
GGACTGT1106.034028E-610.3638426
ATGGCGA5950.010.213160517
Produced by FastQC (version 0.11.5)