##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511877_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 923798 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.12999811647135 31.0 30.0 34.0 27.0 34.0 2 30.316664465608284 31.0 30.0 34.0 27.0 34.0 3 30.37626191007125 31.0 30.0 34.0 27.0 34.0 4 33.98123182773723 35.0 35.0 37.0 30.0 37.0 5 33.585256733614926 35.0 35.0 37.0 28.0 37.0 6 33.61447091247221 36.0 35.0 37.0 29.0 37.0 7 33.430790064494616 35.0 35.0 37.0 28.0 37.0 8 33.29757695946516 35.0 35.0 37.0 28.0 37.0 9 34.58034765175937 37.0 35.0 39.0 27.0 39.0 10 34.28708332341053 37.0 34.0 39.0 27.0 39.0 11 34.37806641711716 37.0 34.0 39.0 27.0 39.0 12 34.06465157967435 37.0 34.0 39.0 27.0 39.0 13 34.08325304882669 37.0 34.0 39.0 27.0 39.0 14 34.781714184269724 38.0 34.0 40.0 26.0 41.0 15 34.67132966297827 38.0 34.0 40.0 25.0 41.0 16 34.51314356601768 38.0 33.0 40.0 25.0 41.0 17 34.399518076462606 38.0 33.0 40.0 25.0 41.0 18 34.54697888499434 38.0 34.0 40.0 25.0 41.0 19 34.6252156856802 38.0 34.0 40.0 25.0 41.0 20 34.49556396528245 38.0 34.0 40.0 24.0 41.0 21 34.354301481492705 38.0 34.0 40.0 24.0 41.0 22 34.16866674316247 38.0 34.0 40.0 22.0 41.0 23 33.94517091398769 38.0 33.0 40.0 19.0 41.0 24 33.673108190318665 38.0 33.0 40.0 18.0 41.0 25 32.742727306185984 37.0 32.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.02132403364373303 1101 2 -0.007765515044052762 1101 3 -0.06914915085774354 1101 4 -0.21841683687890878 1101 5 -0.29125393196220273 1101 6 -0.13653279851675393 1101 7 -0.283608374182883 1101 8 -0.3582597613466305 1101 9 -0.18661766676391522 1101 10 -0.45850563948084044 1101 11 -0.13957661773618213 1101 12 -0.09873436422761017 1101 13 -0.1172850764288711 1101 14 -0.28556279115571925 1101 15 -0.32452325368553403 1101 16 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1103 3 0.5535171833992898 1103 4 0.4191296145394716 1103 5 0.41523706230373136 1103 6 0.26420703046631644 1103 7 0.4747933446011814 1103 8 0.3113246265388625 1103 9 0.3552796525960318 1103 10 0.4230290488133406 1103 11 0.4186640303059761 1103 12 0.5715392080316732 1103 13 0.5254341736132275 1103 14 0.7592811829842034 1103 15 1.0166673906365915 1103 16 0.8422880875990018 1103 17 0.967646220334359 1103 18 0.9918766855706878 1103 19 1.00905047615651 1103 20 1.1711764234518398 1103 21 1.0083738703737168 1103 22 1.0802225558795158 1103 23 0.9410879830747234 1103 24 0.9866056958390885 1103 25 0.7048107824762084 1104 1 -0.0974654983514327 1104 2 -0.10872327205015253 1104 3 -0.0023842749880920167 1104 4 0.0038249402731125315 1104 5 -0.29712692921147976 1104 6 -0.12969617778244213 1104 7 -0.37900166727651197 1104 8 -0.32584746520677754 1104 9 -0.2043300876207681 1104 10 0.2665716024918652 1104 11 -0.12105375367770677 1104 12 -0.3257747038750125 1104 13 0.09502931469580034 1104 14 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-0.7211849292464052 2312 25 -0.8267332406240762 2313 1 0.17318515453709438 2313 2 0.09758039356493953 2313 3 0.06843984669675152 2313 4 0.13904365999887602 2313 5 0.17706389474460593 2313 6 0.0714882953112621 2313 7 -0.0069462897438299365 2313 8 0.19790616770178815 2313 9 0.028465865049987826 2313 10 -0.17068154602888796 2313 11 0.09568288011683279 2313 12 0.09301167696144574 2313 13 0.29890690120249275 2313 14 0.1433942669266699 2313 15 0.24618292637264005 2313 16 0.4169038911968599 2313 17 0.455138541380542 2313 18 0.6745251101105083 2313 19 0.7654753044837079 2313 20 0.4587299274175365 2313 21 0.6237185963314431 2313 22 0.4933067470587247 2313 23 0.36860238966257697 2313 24 0.7526581291036933 2313 25 0.7376530772833689 2314 1 -0.24291875637779015 2314 2 -0.22875209529534857 2314 3 -0.22531458271372173 2314 4 -0.3774541120585724 2314 5 -0.2119442431515992 2314 6 -0.40805291958947976 2314 7 -0.40517167444067326 2314 8 -0.34968912062738156 2314 9 -0.5811065491925547 2314 10 -0.466409354173301 2314 11 -0.27973154978240444 2314 12 -0.7581275470465698 2314 13 -0.4152545859389818 2314 14 -0.6838159579855585 2314 15 -0.3371307206919383 2314 16 -0.08645635122935857 2314 17 -0.6448520906708808 2314 18 -0.32201485844576894 2314 19 -0.6753151874557872 2314 20 -0.40083230427219974 2314 21 -0.2588663872542796 2314 22 -0.506611792518818 2314 23 -0.6110406507662276 2314 24 -0.6889734416976339 2314 25 -0.7198062536007228 2315 1 -0.16170418147900278 2315 2 -0.2650031074410961 2315 3 -0.335330777045705 2315 4 -0.4446565412083672 2315 5 -0.3575960650139507 2315 6 -0.41572498436680405 2315 7 -0.6724996096633475 2315 8 -0.9340696874290018 2315 9 -0.5672765896783858 2315 10 -0.8695024715822086 2315 11 -0.9374076631427286 2315 12 -0.9550627697186371 2315 13 -0.7395015495017319 2315 14 -1.455322030860053 2315 15 -1.0575031903275658 2315 16 -1.0447761892860399 2315 17 -1.065070714152661 2315 18 -1.1198934009558883 2315 19 -0.8480236894800797 2315 20 -0.9380225876730108 2315 21 -0.8412874398858605 2315 22 -1.001773735838654 2315 23 -1.051510286393757 2315 24 -1.090009878944599 2315 25 -0.9653771038031493 2316 1 -0.49522950421981804 2316 2 -0.6219802673219306 2316 3 -0.5224271027656648 2316 4 -0.7313804736810567 2316 5 -0.6840052010616162 2316 6 -0.8472095921125842 2316 7 -1.065071605691152 2316 8 -1.0130647221857032 2316 9 -0.8534692411183045 2316 10 -1.0085948251075365 2316 11 -0.971542717760407 2316 12 -0.7583567121218167 2316 13 -0.865162919908883 2316 14 -1.1320628451599504 2316 15 -1.1940553253821804 2316 16 -1.249736467339666 2316 17 -1.1757320845598116 2316 18 -1.2590135598436731 2316 19 -1.1644298463817648 2316 20 -1.181253968010644 2316 21 -1.2670590386942067 2316 22 -1.1986903197512646 2316 23 -1.0828151523321878 2316 24 -1.0236881311789574 2316 25 -0.9451328138329416 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 21176.0 3 1.0 4 301.0 5 2589.0 6 3307.0 7 2836.0 8 2464.0 9 2253.0 10 2167.0 11 2104.0 12 2071.0 13 1923.0 14 1917.0 15 1885.0 16 1912.0 17 2048.0 18 2135.0 19 2507.0 20 2965.0 21 3766.0 22 4698.0 23 5808.0 24 7119.0 25 8401.0 26 9427.0 27 11056.0 28 12969.0 29 16057.0 30 20514.0 31 26596.0 32 35928.0 33 48813.0 34 65870.0 35 91866.0 36 130213.0 37 183781.0 38 182355.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.467552496041264 21.504547791204814 14.432947114334373 22.59495259841955 2 16.28234743959177 23.083509598418704 39.1757721926222 21.45837076936733 3 19.710477831733776 26.802937438704134 30.6841971946248 22.802387534937292 4 14.32077142405591 19.244250366422097 37.666351301907994 28.768626907614003 5 13.345650106887359 38.808341329128595 35.55147162340996 12.294536940574094 6 31.130706382831246 38.99596253649746 17.664769232102167 12.208561848569126 7 27.385965330082986 32.726418546045785 22.74696416316121 17.140651960710027 8 25.182778053210768 37.93112780066638 20.509029030155943 16.37706511596691 9 26.817115045703922 15.731479196165289 20.527251313094574 36.92415444503622 10 15.032182360212948 30.20021693054109 34.27480899504004 20.492791714205918 11 34.76734091219076 23.160582724794814 24.401221912149627 17.670854450864802 12 22.95242033431551 25.7530325893756 31.907733075845584 19.386814000463303 13 29.011970149318355 22.576905340777962 26.201615504688252 22.209509005215423 14 21.32057008133813 22.420810610111737 28.12400546439806 28.134613844152078 15 23.351317062821092 31.104851926503414 23.838653038867804 21.70517797180769 16 22.543889465012914 28.858040394112134 27.339201860146918 21.258868280728038 17 21.790478004931817 29.090667007289472 27.79547043834258 21.323384549436135 18 21.771859215975788 28.23929040764323 29.489671984568055 20.49917839181293 19 23.43950437396686 27.486871604235297 28.116038672340572 20.957585349457272 20 23.598557260353452 27.785186804907568 28.38163754413844 20.23461839060054 21 23.612452207649373 27.192061382048692 27.58057043390908 21.61491597639285 22 23.414850120754252 27.901972012726016 27.863218071027962 20.81995979549177 23 22.872879970641375 27.937134438034573 27.96398155776082 21.226004033563232 24 23.07090826782995 27.443299594308623 28.476697113374822 21.009095024486605 25 23.202642685300876 27.705536776504267 28.187019140196146 20.904801397998714 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 95.0 1 95.0 2 213.0 3 331.0 4 331.0 5 331.0 6 877.5 7 1424.0 8 1424.0 9 1424.0 10 1979.5 11 2535.0 12 2535.0 13 2535.0 14 4008.0 15 5481.0 16 5481.0 17 5481.0 18 9569.5 19 13658.0 20 13658.0 21 13658.0 22 20851.5 23 28045.0 24 28045.0 25 28045.0 26 39096.0 27 50147.0 28 50147.0 29 50147.0 30 63033.5 31 75920.0 32 75920.0 33 75920.0 34 84238.5 35 92557.0 36 92557.0 37 92557.0 38 99098.0 39 105639.0 40 105639.0 41 105639.0 42 110240.5 43 114842.0 44 114842.0 45 114842.0 46 115627.5 47 116413.0 48 116413.0 49 116413.0 50 111593.5 51 106774.0 52 106774.0 53 106774.0 54 94659.0 55 82544.0 56 82544.0 57 82544.0 58 70262.0 59 57980.0 60 57980.0 61 57980.0 62 46940.5 63 35901.0 64 35901.0 65 35901.0 66 27600.0 67 19299.0 68 19299.0 69 19299.0 70 14118.5 71 8938.0 72 8938.0 73 8938.0 74 6257.0 75 3576.0 76 3576.0 77 3576.0 78 2396.5 79 1217.0 80 1217.0 81 1217.0 82 781.5 83 346.0 84 346.0 85 346.0 86 230.5 87 115.0 88 115.0 89 115.0 90 65.5 91 16.0 92 16.0 93 16.0 94 10.0 95 4.0 96 4.0 97 4.0 98 2.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3984637334135817 2 0.0 3 0.0 4 0.0 5 0.04286651410806259 6 0.04827895275807049 7 0.0 8 0.0 9 0.0028144680980041093 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.004005204601005848 20 0.0 21 0.0010824877300015805 22 0.002273224233003319 23 0.005304189877007744 24 0.04719646502806891 25 0.020783764416030345 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 923798.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.34255315546254 #Duplication Level Percentage of deduplicated Percentage of total 1 85.05247931275296 64.08070943624968 2 10.630163462439231 16.01807311440167 3 2.3241036087873113 5.25311699051581 4 0.8203227979320987 2.4722085603134767 5 0.37932810472311135 1.4289773946730941 6 0.21289624905388246 0.9624088176564439 7 0.1297196822240905 0.6841388437294773 8 0.08625360444840094 0.519885342240311 9 0.06246365297450516 0.42355539850644186 >10 0.27146595322819633 3.526880306479762 >50 0.017067782700995144 0.8913720350565818 >100 0.011711567501359833 1.9272907516179179 >500 0.0011566978479122432 0.6662508107017447 >1k 8.675233859341823E-4 1.1451321978575442 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3240 0.35072602452051205 No Hit TATCAACGCAGAGTACTTTTTTTTT 2143 0.23197712053933867 No Hit GGTATCAACGCAGAGTACTTTTTTT 1988 0.2151985607243142 No Hit CTGTAGGACGTGGAATATGGCAAGA 1074 0.11625918220216974 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1062 0.11496019692616784 No Hit CTTTAGGACGTGAAATATGGCGAGG 1005 0.10879001686515882 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 984 0.10651679263215552 No Hit GTCCTACAGTGGACATTTCTAAATT 960 0.10391882208015171 No Hit GTCCTAAAGTGTGTATTTCTCATTT 958 0.1037023245341514 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 1.0824877300015805E-4 7 0.0 0.0 0.0 0.0 1.0824877300015805E-4 8 0.0 0.0 0.0 0.0 1.0824877300015805E-4 9 0.0 0.0 0.0 0.0 1.0824877300015805E-4 10 0.0 0.0 0.0 0.0 1.0824877300015805E-4 11 0.0 0.0 0.0 0.0 1.0824877300015805E-4 12 0.0 0.0 0.0 0.0 1.0824877300015805E-4 13 0.0 0.0 0.0 0.0 1.0824877300015805E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATACTG 145 0.0 14.421111 5 CCATTAG 40 0.0051338063 14.309182 1 GGTATCA 560 0.0 12.776055 1 ATAGGAC 60 4.07176E-4 12.673096 3 ACTGCCC 105 2.7354145E-7 11.7551365 8 ACATTCG 65 8.01667E-4 11.692541 18 CTAAACT 75 2.0623318E-4 11.405787 4 TCGAACT 75 2.0716935E-4 11.400227 19 GGCGAGG 270 0.0 11.259484 19 GTATACT 60 0.005850454 11.088959 4 TGGCGAG 550 0.0 11.054766 18 CTCTTAG 140 7.8052835E-9 10.902234 1 TAAGCTG 70 0.0014841118 10.862655 5 GACGTGA 300 0.0 10.76047 7 CAATACT 80 3.749921E-4 10.692925 4 GTATCAA 1240 0.0 10.61649 1 ATTTAGA 235 0.0 10.554291 1 TAGGACC 190 9.094947E-12 10.50533 4 GGACTGT 110 6.034028E-6 10.363842 6 ATGGCGA 595 0.0 10.2131605 17 >>END_MODULE