FastQCFastQC Report
Thu 26 May 2016
SRR1511876_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511876_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1122333
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT36120.32182961741301375No Hit
CTGTAGGACGTGGAATATGGCAAGA35760.3186220132527512No Hit
GTCCTAAAGTGTGTATTTCTCATTT34320.3057915966117008No Hit
CTTTAGGACGTGAAATATGGCGAGG30180.26890414876868096No Hit
GTATCAACGCAGAGTACTTTTTTTT28250.2517078264650509No Hit
TATCAACGCAGAGTACTTTTTTTTT17570.15654890304392724No Hit
GGTATCAACGCAGAGTACTTTTTTT17390.15494510096379596No Hit
CTGAAGGACCTGGAATATGGCGAGA15180.13525397542440615No Hit
GTCCTACAGTGTGCATTTCTCATTT14420.12848236664162951No Hit
ATTTAGAAATGTCCACTGTAGGACG13450.1198396554320331No Hit
TTTCTAAATTTTCCACCTTTTTCAG13230.1178794528896504No Hit
GTCCTTCAGTGTGCATTTCTCATTT12480.11119694422243664No Hit
CTGTAGGACCTGGAATATGGCGAGA11710.10433623532409722No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGT400.005282165514.2482625
TAGGACC5000.013.8683094
CGGTAGG655.3970754E-513.1674951
GGTATCA6150.012.8344411
CCAACGA604.094662E-412.66512219
GGCGAGG8200.012.16315119
TATTGCG550.003070334712.08943616
ATTGCGA550.003070334712.08943617
GGACCTA802.8682754E-511.8735526
TAGGGAC658.0285815E-411.6908835
TTAACCT907.465407E-611.6096964
CCTACAC1354.7293724E-911.2578873
ATAGGAC2000.010.9236673
GGCGTGC700.001492359810.855828
GGACCTG11200.010.855826
AGGACCT12000.010.7653535
GCGCCAC803.7731646E-410.68619613
GTAGGAC19650.010.5864953
GCCTACA1355.592301E-810.5665082
AGGACGT19650.010.5381555