FastQCFastQC Report
Thu 26 May 2016
SRR1511876_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511876_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1122333
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA36430.32459172099546213No Hit
GTCCTACAGTGGACATTTCTAAATT34990.31176130435441174No Hit
GTATCAACGCAGAGTACTTTTTTTT34570.30801909950077205No Hit
GTCCTAAAGTGTGTATTTCTCATTT33500.2984853871355471No Hit
CTTTAGGACGTGAAATATGGCGAGG32260.28743697280575375No Hit
TATCAACGCAGAGTACTTTTTTTTT22180.19762405631840105No Hit
GGTATCAACGCAGAGTACTTTTTTT21940.19548565354489267No Hit
CTGAAGGACCTGGAATATGGCGAGA17280.1539649996926046No Hit
GTCCTACAGTGTGCATTTCTCATTT15140.1348975749621547No Hit
ATTTAGAAATGTCCACTGTAGGACG14160.12616576363699544No Hit
TTTCTAAATTTTCCACCTTTTTCAG14020.12491836201911555No Hit
GAATATGGCAAGAAAACTGAAAATC12920.11511734930720204No Hit
CTGTAGGACCTGGAATATGGCGAGA12110.1079002399466112No Hit
GTCCTTCAGTGTGCATTTCTCATTT11730.10451443555522291No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6350.013.6693631
CCACCTT4900.012.20861513
TAGGACC4950.012.0955344
CACCTTT5550.011.63423514
AGGACGA907.4235977E-611.6155535
GGACGAG907.4568343E-611.6108936
TTCCACC5350.011.35870311
AGGACCT10750.011.3159545
GTATCAA14000.011.3099491
CTAGGAC1604.5474735E-1111.2855653
AAAGGCG600.005857230611.0875745
CCCCTAT700.001441353810.9011551
TTTAGGC700.001486092710.8612963
GTGCGCC700.001497186310.85161811
ACCTTTT5700.010.82830315
GGTCTAC1158.359111E-710.7826651
TCCACCT5550.010.77829612
TCTAAGC1158.763054E-710.7432383
TAGACTG1252.2251879E-710.644075
GGCGAGG9400.010.61008419
Produced by FastQC (version 0.11.5)