##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511876_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1122333 Sequences flagged as poor quality 0 Sequence length 25 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.715549663067915 31.0 31.0 34.0 27.0 34.0 2 30.928792078643326 31.0 31.0 34.0 27.0 34.0 3 31.01588120459792 31.0 31.0 34.0 27.0 34.0 4 34.62580178966492 37.0 35.0 37.0 32.0 37.0 5 34.294985534596236 37.0 35.0 37.0 30.0 37.0 6 34.29371674895062 37.0 35.0 37.0 30.0 37.0 7 34.151106667985346 37.0 35.0 37.0 30.0 37.0 8 34.03326641914654 37.0 35.0 37.0 30.0 37.0 9 35.43613704666975 38.0 35.0 39.0 30.0 39.0 10 35.18675206021742 38.0 35.0 39.0 30.0 39.0 11 35.2302890496849 38.0 35.0 39.0 30.0 39.0 12 35.00454766989833 38.0 35.0 39.0 29.0 39.0 13 35.02055361465804 38.0 35.0 39.0 29.0 39.0 14 35.867966102752035 39.0 36.0 40.0 28.0 41.0 15 35.78712200389724 39.0 36.0 40.0 28.0 41.0 16 35.64232362409374 39.0 36.0 40.0 27.0 41.0 17 35.56043170787993 38.0 36.0 40.0 27.0 41.0 18 35.710934277081755 39.0 36.0 40.0 27.0 41.0 19 35.80898360825174 39.0 36.0 40.0 27.0 41.0 20 35.70216147970344 39.0 36.0 40.0 27.0 41.0 21 35.59632212542979 39.0 36.0 40.0 27.0 41.0 22 35.44839454956773 39.0 35.0 40.0 27.0 41.0 23 35.26774584726636 39.0 35.0 40.0 27.0 41.0 24 35.069385823993414 39.0 35.0 40.0 27.0 41.0 25 34.23227865526542 38.0 34.0 40.0 24.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.05096704031952015 1101 2 0.004385276829669493 1101 3 -0.08660741245924442 1101 4 -0.07936133540649593 1101 5 -0.2537963200908564 1101 6 -0.20184635476989854 1101 7 -0.06923883975537137 1101 8 -0.2320616860198399 1101 9 -0.27293314374614397 1101 10 -0.34629987018764297 1101 11 -0.08232217363809013 1101 12 0.021309077385815556 1101 13 0.1332708601472703 1101 14 -0.3605810859829859 1101 15 -0.38106067937849986 1101 16 -0.13586135960221668 1101 17 -0.09640986216567171 1101 18 -0.26908198952567375 1101 19 -0.15451433547281823 1101 20 -0.05858632907708028 1101 21 -0.35357318434036245 1101 22 -0.5591656579109738 1101 23 -0.679041528748435 1101 24 -0.3748632537864154 1101 25 -0.4137610055435701 1102 1 0.16578688638204753 1102 2 0.004564349596950734 1102 3 0.05182949474160381 1102 4 0.20303490103026434 1102 5 0.09731377930970808 1102 6 0.1872909090589161 1102 7 0.2017498543792371 1102 8 0.2668213855333903 1102 9 0.2812090521716044 1102 10 0.3420239783592862 1102 11 0.3857937683908972 1102 12 0.38196618338778876 1102 13 0.46656170163751653 1102 14 0.5201458280469069 1102 15 0.4608544886919148 1102 16 0.5857563655666098 1102 17 0.5262625712511309 1102 18 0.6101121830215632 1102 19 0.5847845491163 1102 20 0.6842879405943734 1102 21 0.8863372792759989 1102 22 0.7680579554132478 1102 23 0.703121002551363 1102 24 0.7573000362962858 1102 25 0.8180441390045772 1103 1 0.7568111736048593 1103 2 0.6651612000629541 1103 3 0.8045525102138029 1103 4 0.6710666718396823 1103 5 0.6988049872432285 1103 6 0.7226354754240987 1103 7 0.7629251906815142 1103 8 0.9183102978881976 1103 9 0.8090516672948809 1103 10 0.8833958161996023 1103 11 0.9336933138663142 1103 12 0.9253801680533229 1103 13 0.958611653430367 1103 14 1.054056116830715 1103 15 1.2255163499471422 1103 16 1.4939653550528575 1103 17 1.3279337525559356 1103 18 1.4699395555021653 1103 19 1.5230068529590994 1103 20 1.3881463492992054 1103 21 1.70542639364173 1103 22 1.4500413354233501 1103 23 1.3706165261728671 1103 24 1.665113268012007 1103 25 1.3004398049519565 1104 1 0.2571661967268781 1104 2 0.23173076638855505 1104 3 0.3321068560609426 1104 4 0.30869457119518273 1104 5 0.4386331196395403 1104 6 0.19322044429129903 1104 7 0.25552796532376476 1104 8 0.1985455234644249 1104 9 0.39467232053742407 1104 10 0.4950824491239061 1104 11 0.7544594355573153 1104 12 0.5567787770909405 1104 13 0.5362843403181756 1104 14 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-0.8548153956683748 2314 10 -0.7287525183763748 2314 11 -0.6435575304005994 2314 12 -0.941530135545392 2314 13 -0.8020019877637878 2314 14 -0.9718808423239764 2314 15 -0.8432294118498262 2314 16 -0.8592637761197324 2314 17 -0.9534846158581942 2314 18 -0.6788723981702702 2314 19 -1.0373949202544424 2314 20 -1.1092306036478874 2314 21 -0.8178132971929557 2314 22 -0.640523792051674 2314 23 -0.8089112241467902 2314 24 -1.1478854061262709 2314 25 -1.0301653345334145 2315 1 -0.523602181142067 2315 2 -0.4561891803904672 2315 3 -0.5134885536297098 2315 4 -0.6259739064630949 2315 5 -0.4671990125258887 2315 6 -0.5523749653993519 2315 7 -0.6214040670523318 2315 8 -0.9205419585180579 2315 9 -0.8236113029241565 2315 10 -0.7892062746813977 2315 11 -0.7980242100796744 2315 12 -0.7817343618379908 2315 13 -0.8189241085120713 2315 14 -1.0149373538348172 2315 15 -0.8867199555971936 2315 16 -1.0847525118274888 2315 17 -0.8674743223599393 2315 18 -0.7451303725495251 2315 19 -0.856888874621994 2315 20 -0.9122981072177367 2315 21 -0.9637200393315837 2315 22 -0.9389684670668288 2315 23 -0.6409150662309671 2315 24 -0.8320974193167388 2315 25 -0.9217112084021082 2316 1 -0.36031399432251376 2316 2 -0.3556605259556669 2316 3 -0.3762688099891385 2316 4 -0.575591478334097 2316 5 -0.5546387392268599 2316 6 -0.468998296218345 2316 7 -0.6964184193701968 2316 8 -0.5727943070206507 2316 9 -0.7212631864888905 2316 10 -0.6651629886055872 2316 11 -0.8911025312575163 2316 12 -0.8748410014905446 2316 13 -0.6781182162098034 2316 14 -0.8151241270476035 2316 15 -0.9073113483255213 2316 16 -0.8973466385758329 2316 17 -0.8334895782945964 2316 18 -0.87325538059455 2316 19 -1.271617442041844 2316 20 -1.2926821572230125 2316 21 -1.1745510835882058 2316 22 -1.2477891570593371 2316 23 -1.1431824750132975 2316 24 -1.260823680068107 2316 25 -1.1857146941489063 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 18540.0 3 0.0 4 344.0 5 2336.0 6 3056.0 7 2674.0 8 2319.0 9 2108.0 10 2018.0 11 1915.0 12 1912.0 13 1768.0 14 1755.0 15 1723.0 16 1762.0 17 1856.0 18 1958.0 19 2260.0 20 2845.0 21 3483.0 22 4366.0 23 5475.0 24 6687.0 25 7779.0 26 9184.0 27 10612.0 28 11986.0 29 14960.0 30 19032.0 31 25143.0 32 34165.0 33 46628.0 34 65911.0 35 97161.0 36 152727.0 37 251729.0 38 302156.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.840765072110266 21.858742978219283 14.554838340220153 23.7456536094503 2 14.450702242560808 22.394333945451127 42.1426617590323 21.01230205295576 3 18.386699847549703 26.97898039173757 31.27966476972521 23.354654990987523 4 12.745771531265676 19.380700736768855 39.68822087562248 28.185306856342997 5 12.203390434862449 39.25359443681288 36.880004778022524 11.663010350302146 6 29.62160697364914 39.785064132220505 18.792001946831185 11.801326947299174 7 26.502205673360756 32.631224422698075 23.996888623964548 16.86968127997662 8 23.531875120842034 39.68323126915095 21.278176797795307 15.506716812211705 9 26.146213503649633 15.679263372490876 21.679509295164234 36.49501382869526 10 14.588451021220974 30.31301761598385 35.65038183854524 19.44814952424993 11 32.86903263113532 22.91966822680969 26.725312362730136 17.485986779324854 12 23.053407500269525 25.617263325590535 33.27648745960424 18.052841714535703 13 28.551419231190746 23.381919626349756 26.66837738888547 21.39828375357403 14 19.625191453873317 24.018807252393007 28.92154111123882 27.434460182494856 15 21.451922023142863 34.01619661900702 23.969089387908937 20.562791969941184 16 20.378176530494958 29.436807079538784 30.11583906024326 20.069177329722994 17 20.338616079185055 30.515809479004897 29.328639539245483 19.81693490256457 18 20.080671244630604 28.258547151335655 32.510939266688226 19.14984233734551 19 22.846994939661773 27.68483511352569 28.940340759736856 20.52782918707569 20 22.875118169028266 28.64149944802478 29.666685377690932 18.816697005256017 21 21.75123271657409 27.81716055520806 28.47401538601359 21.95759134220426 22 22.358003196966266 30.029563918831986 28.55974352988558 19.052689354316172 23 20.610061234236994 29.675704478867416 29.67151645625566 20.04271783063993 24 22.346940412794233 28.05125252046232 30.264713170942166 19.33709389580128 25 21.498809379522754 28.893845846730787 29.768452835031333 19.83889193871513 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 111.0 1 111.0 2 238.0 3 365.0 4 365.0 5 365.0 6 923.0 7 1481.0 8 1481.0 9 1481.0 10 2239.0 11 2997.0 12 2997.0 13 2997.0 14 5075.5 15 7154.0 16 7154.0 17 7154.0 18 12840.5 19 18527.0 20 18527.0 21 18527.0 22 29090.0 23 39653.0 24 39653.0 25 39653.0 26 56294.0 27 72935.0 28 72935.0 29 72935.0 30 92861.0 31 112787.0 32 112787.0 33 112787.0 34 123773.0 35 134759.0 36 134759.0 37 134759.0 38 139674.5 39 144590.0 40 144590.0 41 144590.0 42 145826.0 43 147062.0 44 147062.0 45 147062.0 46 144282.5 47 141503.0 48 141503.0 49 141503.0 50 129695.5 51 117888.0 52 117888.0 53 117888.0 54 98263.5 55 78639.0 56 78639.0 57 78639.0 58 64506.5 59 50374.0 60 50374.0 61 50374.0 62 39362.5 63 28351.0 64 28351.0 65 28351.0 66 21290.5 67 14230.0 68 14230.0 69 14230.0 70 10077.5 71 5925.0 72 5925.0 73 5925.0 74 4008.5 75 2092.0 76 2092.0 77 2092.0 78 1373.5 79 655.0 80 655.0 81 655.0 82 420.5 83 186.0 84 186.0 85 186.0 86 124.5 87 63.0 88 63.0 89 63.0 90 33.5 91 4.0 92 4.0 93 4.0 94 3.0 95 2.0 96 2.0 97 2.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.42224544765234556 2 0.0 3 0.0 4 0.0 5 0.04722306124831044 6 0.04544105893705344 7 0.0 8 0.0 9 0.0025839033513226466 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.006771608782776591 20 0.0 21 0.0013365017334427483 22 0.0016929021956941478 23 0.0075735098228422405 24 0.04588655951486769 25 0.020403926463892625 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1122333.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.11584873882549 #Duplication Level Percentage of deduplicated Percentage of total 1 85.38623249804218 62.43086859705037 2 10.893491620368604 15.929737711050679 3 2.1141448299346117 4.637324807924071 4 0.6325009862749932 1.8498338575856137 5 0.30084310771978384 1.0998199579078949 6 0.1499865642851002 0.6579836968275309 7 0.10091662812646625 0.5165023442128907 8 0.06649587466758436 0.38895218511607965 9 0.046514774991951946 0.30608705271888553 >10 0.2598616223680443 3.4172833761225494 >50 0.024213201924541603 1.2479604865422518 >100 0.019396690336262554 2.8260864175962483 >500 0.003437382429010267 1.6910725712876045 >1k 0.00196421853086301 3.000486938057433 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGTAGGACGTGGAATATGGCAAGA 3643 0.32459172099546213 No Hit GTCCTACAGTGGACATTTCTAAATT 3499 0.31176130435441174 No Hit GTATCAACGCAGAGTACTTTTTTTT 3457 0.30801909950077205 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3350 0.2984853871355471 No Hit CTTTAGGACGTGAAATATGGCGAGG 3226 0.28743697280575375 No Hit TATCAACGCAGAGTACTTTTTTTTT 2218 0.19762405631840105 No Hit GGTATCAACGCAGAGTACTTTTTTT 2194 0.19548565354489267 No Hit CTGAAGGACCTGGAATATGGCGAGA 1728 0.1539649996926046 No Hit GTCCTACAGTGTGCATTTCTCATTT 1514 0.1348975749621547 No Hit ATTTAGAAATGTCCACTGTAGGACG 1416 0.12616576363699544 No Hit TTTCTAAATTTTCCACCTTTTTCAG 1402 0.12491836201911555 No Hit GAATATGGCAAGAAAACTGAAAATC 1292 0.11511734930720204 No Hit CTGTAGGACCTGGAATATGGCGAGA 1211 0.1079002399466112 No Hit GTCCTTCAGTGTGCATTTCTCATTT 1173 0.10451443555522291 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 8.910011556284988E-5 0.0 0.0 0.0 6 0.0 8.910011556284988E-5 0.0 0.0 0.0 7 0.0 8.910011556284988E-5 0.0 0.0 0.0 8 0.0 8.910011556284988E-5 0.0 0.0 0.0 9 0.0 8.910011556284988E-5 0.0 8.910011556284988E-5 0.0 10 0.0 8.910011556284988E-5 0.0 8.910011556284988E-5 0.0 11 0.0 8.910011556284988E-5 0.0 8.910011556284988E-5 0.0 12 0.0 8.910011556284988E-5 0.0 8.910011556284988E-5 0.0 13 0.0 8.910011556284988E-5 0.0 8.910011556284988E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 635 0.0 13.669363 1 CCACCTT 490 0.0 12.208615 13 TAGGACC 495 0.0 12.095534 4 CACCTTT 555 0.0 11.634235 14 AGGACGA 90 7.4235977E-6 11.615553 5 GGACGAG 90 7.4568343E-6 11.610893 6 TTCCACC 535 0.0 11.358703 11 AGGACCT 1075 0.0 11.315954 5 GTATCAA 1400 0.0 11.309949 1 CTAGGAC 160 4.5474735E-11 11.285565 3 AAAGGCG 60 0.0058572306 11.087574 5 CCCCTAT 70 0.0014413538 10.901155 1 TTTAGGC 70 0.0014860927 10.861296 3 GTGCGCC 70 0.0014971863 10.851618 11 ACCTTTT 570 0.0 10.828303 15 GGTCTAC 115 8.359111E-7 10.782665 1 TCCACCT 555 0.0 10.778296 12 TCTAAGC 115 8.763054E-7 10.743238 3 TAGACTG 125 2.2251879E-7 10.64407 5 GGCGAGG 940 0.0 10.610084 19 >>END_MODULE