FastQCFastQC Report
Thu 26 May 2016
SRR1511875_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511875_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1056307
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT26000.24614056330214606No Hit
GGTATCAACGCAGAGTACTTTTTTT15480.1465483046122008No Hit
TATCAACGCAGAGTACTTTTTTTTT14720.13935342660798422No Hit
GTCCTACAGTGGACATTTCTAAATT12630.11956751209638865No Hit
CTGTAGGACGTGGAATATGGCAAGA11800.11170994796020477No Hit
GTCCTAAAGTGTGTATTTCTCATTT11610.10991122845915062No Hit
CTTTAGGACGTGAAATATGGCGAGG10820.10243234211266233No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5700.013.6823151
TACACGG604.0947416E-412.6649635
ACCGTGC550.003070419712.0892828
GGCGTGC951.0404037E-611.9983858
GTATAGA1354.634785E-911.272151
AATGCCG600.00587943111.08184218
CCTACAG7150.010.893643
TAACTCC700.001492391310.8556814
TCCTACA7250.010.6259462
GTATAAC909.397847E-510.5676411
CTAGAGT909.520511E-510.5541354
AGGACGT6950.010.5237645
GTCCTAC6800.010.4899391
ATCCTAC1002.3662982E-510.4619651
GTCTAGG1002.3662982E-510.4619651
TAAGAGT1106.0478287E-610.3622424
CTACAGT7800.010.35117154
GTATCAA12050.010.3396251
GTAGGCT750.002649325410.131973
TACAGTG8900.010.0323585
Produced by FastQC (version 0.11.5)