FastQCFastQC Report
Thu 26 May 2016
SRR1511875_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511875_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1056307
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT30500.2887418146429021No Hit
GGTATCAACGCAGAGTACTTTTTTT19700.1864988114250876No Hit
TATCAACGCAGAGTACTTTTTTTTT19180.18157600015904468No Hit
CTGTAGGACGTGGAATATGGCAAGA13010.12316495109849694No Hit
GTCCTACAGTGGACATTTCTAAATT12440.1177687925953345No Hit
GTCCTAAAGTGTGTATTTCTCATTT11270.10669246724673792No Hit
CTTTAGGACGTGAAATATGGCGAGG11170.10574577277249891No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCGG250.006051258618.98887610
GGTATCA6600.012.4297891
CCTACAC1103.781679E-812.0973
CTAATAC802.8495928E-511.8809823
CCTATAC907.412429E-611.6169613
CTTATAC1053.4413406E-610.8626133
GTGGTAT2400.010.7315331
GACGAAA803.769124E-410.68731419
TAGGACC2600.010.6014924
TATAGTC1002.3830877E-510.4552655
TCTATAC1002.3830877E-510.4552653
GTATCAA14250.010.3759071
GTCTTAG1201.4432771E-610.3340691
GGCGAGG2500.010.25982219
GTCCTAA4200.010.2205081
GTATAGA1502.2882887E-810.1750821
TAGGACT1502.4201654E-810.1384374
TACACCG750.002635334210.1384375
TAGAACA2450.010.0867114
GTGCTAT1053.919621E-59.9933841
Produced by FastQC (version 0.11.5)