FastQCFastQC Report
Thu 26 May 2016
SRR1511874_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511874_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences812315
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT19820.243994017099278No Hit
GTCCTACAGTGGACATTTCTAAATT18490.22762105833328203No Hit
GTCCTAAAGTGTGTATTTCTCATTT17280.21272535900481956No Hit
CTGTAGGACGTGGAATATGGCAAGA17150.2111249946141583No Hit
CTTTAGGACGTGAAATATGGCGAGG14850.1828108553947668No Hit
GGTATCAACGCAGAGTACTTTTTTT11570.14243243076885198No Hit
TATCAACGCAGAGTACTTTTTTTTT11230.13824686236250716No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC3100.013.7889814
CGTGCGC655.451207E-513.15256910
GTGCGCC655.451207E-513.15256911
GGCGAGG3900.012.90900119
GGTATCA3550.012.3200381
AGGACCT6400.012.3191165
ATGTCGA550.003068510412.08973417
TGTAGGA9300.011.9614952
GTGCTAG1052.6845555E-711.771631
GTCCTAA5000.011.5995841
CACCTTT2550.011.54789714
GTATCAA8700.011.47499751
GGACCTG6000.011.3988926
CTTAGAC752.0733639E-411.3988923
CTGTAGG9200.011.2647421
GACCTAC600.00583554911.0924981
AGGACGT10050.011.0586265
GTAGGAC9700.010.9680053
GGACGTG9900.010.9383326
CCACCTT3050.010.9005813
Produced by FastQC (version 0.11.5)