FastQCFastQC Report
Thu 26 May 2016
SRR1511874_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511874_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences812315
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT26360.32450465644485205No Hit
TATCAACGCAGAGTACTTTTTTTTT17190.21161741442666948No Hit
GTCCTACAGTGGACATTTCTAAATT17140.2110018896610305No Hit
CTGTAGGACGTGGAATATGGCAAGA16720.2058314816296634No Hit
GGTATCAACGCAGAGTACTTTTTTT16600.20435422219212987No Hit
GTCCTAAAGTGTGTATTTCTCATTT15720.19352098631688447No Hit
CTTTAGGACGTGAAATATGGCGAGG14770.1818260157697445No Hit
ACGCAGAGTACTTTTTTTTTTTTTT8590.10574715473677083No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGTT402.7688508E-416.61729811
CCGGTTT456.7811966E-414.77093112
TGCACTG1001.4327998E-712.3541635
GGTATCA5950.012.3438881
TGGTATC2250.012.2485722
GTGCGCC701.0943884E-412.20862711
TCCAACG550.003069850612.08902818
TGTCGAG550.003069850612.08902818
TGCGCCA550.003076936812.08530812
GTGGTAT2450.012.0690711
TAGGACC2600.012.0617574
AATCCCG907.4610944E-611.60930419
GGCGAGG3900.011.44693819
GTATAAG1001.8342671E-611.4461511
AATGCCG600.005891852511.0781998
GACTGAT600.005891852511.0781997
TGCCGGT600.005891852511.07819910
AACAGTG2250.010.9726927
TGTAGGA9950.010.8880922
GTGTGCG700.001495846310.8521139
Produced by FastQC (version 0.11.5)