FastQCFastQC Report
Thu 26 May 2016
SRR1511873_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511873_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1315902
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT29170.22167304252140355No Hit
GTCCTAAAGTGTGTATTTCTCATTT21230.16133420269898519No Hit
GTCCTACAGTGGACATTTCTAAATT20180.15335488509022707No Hit
CTTTAGGACGTGAAATATGGCGAGG19090.14507159347732582No Hit
CTGTAGGACGTGGAATATGGCAAGA18860.14332374295350261No Hit
TATCAACGCAGAGTACTTTTTTTTT18790.14279178844625207No Hit
GGTATCAACGCAGAGTACTTTTTTT18570.14111993142346466No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCTAA902.246452E-914.795891
GCGTAGA400.00523581214.2674651
CCAACGA655.4586195E-513.15195219
CTAGTAC701.0921222E-412.2125273
GTGGTAT2750.012.1057281
TTCAACG550.003039998712.1057272
TTGGACT1052.7235365E-711.7602114
TCCAACG1157.082963E-811.56355218
CGTTTCC1001.9319923E-611.39835914
AGGACGT12250.011.24327855
GACGTGG6550.011.166337
GGTATCA8350.011.1633661
TGTCGAG600.00588114711.081737518
GGACGTG12050.011.0357556
CTTATAC1303.2699973E-810.959963
TAGGACC3400.010.8954894
AGGACAT3300.010.6499825
GTAGGAC10950.010.5829523
GACCTAC909.3942086E-510.5684931
ACGTGGA7200.010.4221118
Produced by FastQC (version 0.11.5)