FastQCFastQC Report
Thu 26 May 2016
SRR1511873_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511873_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1315902
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT36130.2745645192423144No Hit
GGTATCAACGCAGAGTACTTTTTTT22130.1681736177922064No Hit
GTCCTACAGTGGACATTTCTAAATT22030.16741368278184848No Hit
TATCAACGCAGAGTACTTTTTTTTT21770.16543785175491793No Hit
GTCCTAAAGTGTGTATTTCTCATTT21550.1637659947321305No Hit
CTGTAGGACGTGGAATATGGCAAGA19970.15175902156847546No Hit
CTTTAGGACGTGAAATATGGCGAGG19710.14978319054154487No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGCGA500.001505244213.29305810
GGTATCA6900.013.1402871
ACTATCG550.003080035812.08459811
GTATCAA16850.011.498111
ACGAAAT752.0834315E-411.39405112
TAACCCC600.005854487411.0885034
ACTCCGA600.005897720311.0775488
ACTGATC1553.1468517E-1011.02658
GGACGTG11300.010.7591246
GACGTGA6800.010.7517397
AGGACGT11250.010.7294465
GTATAGA1252.1001506E-710.6892771
CCAACGT803.778774E-410.68476419
TCCAACG1252.2473068E-710.63687118
TTAGGAC7700.010.4919043
TAGGACC3000.010.4548754
GCCGGTT1002.411458E-510.44454611
CCGGTTT1002.411458E-510.44454612
GGCGAGG5750.010.40603119
TCTAGAC1106.0082402E-610.368473
Produced by FastQC (version 0.11.5)