FastQCFastQC Report
Thu 26 May 2016
SRR1511872_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511872_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1314278
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT22430.17066404520200443No Hit
GGTATCAACGCAGAGTACTTTTTTT13300.10119624615187958No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6050.012.7337731
TCCGGAC752.0759359E-411.398473
AGCGCAC600.0058807111.08184610
TCTAGAC1658.0035534E-1110.9379263
TTAGTAC700.001492807110.8556863
TTGGACA1803.45608E-1110.5541394
TAAGACT1553.6652636E-910.4179574
GTATCAA12150.09.8632881
CTAGACT1752.575689E-99.7701174
TAGGACG1856.493792E-109.7554484
GTATAAT1951.6007107E-109.7549011
GGCCTAT800.0044716599.5110281
TTAGACC800.0045196659.4987264
GTGGCCG800.0045196659.4987269
TGATCAC1401.0594122E-69.49872510
TAGAGGT1401.0594122E-69.4987254
TAGACTG2451.8189894E-129.3048735
GTCCAAG2151.03682396E-109.2898411
TAGACAG1554.2632746E-79.1923155
ACTGATC1451.7075399E-69.1711838
Produced by FastQC (version 0.11.5)