FastQCFastQC Report
Thu 26 May 2016
SRR1511872_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511872_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1314278
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT26450.20125118125693345No Hit
TATCAACGCAGAGTACTTTTTTTTT16770.12759857503511432No Hit
GGTATCAACGCAGAGTACTTTTTTT16000.12173984499474237No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6750.014.1283611
TCTAGAC907.426612E-611.615513
CTAACAC1406.7484507E-1011.5400833
GTATTAA1407.854396E-910.8990211
TTTAGAC803.7566677E-410.6915473
TAGAACC1252.2265704E-710.644034
TGGTATC2150.010.6086682
GTATAGA2250.010.5962711
GTCCTAA1105.7704037E-610.4036111
GTATCAA14750.010.3448331
GCATAGT856.3622295E-410.0976231
GCTTATA856.568586E-410.06263352
CCTATAC856.568586E-410.06263353
TATGGTT2002.5465852E-119.9787785
GTTCTAT1451.5270962E-79.8654941
GTGTATA2600.09.5366441
GTGGTAT2800.09.5366431
TTATGGT1304.2145366E-69.5035994
TTAGACA1002.7436524E-49.5035974
CTTATAC1002.7436524E-49.5035973
Produced by FastQC (version 0.11.5)