FastQCFastQC Report
Thu 26 May 2016
SRR1511871_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511871_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences153070
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT12790.8355654275821519No Hit
GGTATCAACGCAGAGTACTTTTTTT7730.5049977134644281No Hit
TATCAACGCAGAGTACTTTTTTTTT7210.47102632782387144No Hit
ACGCAGAGTACTTTTTTTTTTTTTT4040.26393153459201674No Hit
GTACTTTTTTTTTTTTTTTTTTTTT3340.2182008231528059No Hit
GAGTACTTTTTTTTTTTTTTTTTTT2880.18814921277846736No Hit
GCAGAGTACTTTTTTTTTTTTTTTT1570.10256745279937285No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGAAGC250.00601258518.9973854
GGTATCA1200.016.6444631
TTACACC350.002160185516.2834744
GTATCAA2800.010.5301711
TATCAAC4101.8189894E-126.95935732
ATCAACG4400.06.908143
TCAACGC4600.06.60778674
CAACGCA4650.06.53673555
AACGCAG4701.8189894E-126.46719556
CATGGGG3151.118442E-66.03091674
TACATGG3501.8377068E-75.978422
ACATGGG3801.5240948E-75.7492093
ACGCAGA5601.4915713E-105.42782457
CGCAGAG5702.419256E-105.33268
GTACATG3754.1462226E-65.3262281
GAGTACA2500.00211826945.31926812
AGAGTAC5803.8926373E-105.240658311
AGTACAT2600.003461575.11468113
GCAGAGT6009.731593E-105.06596959
Produced by FastQC (version 0.11.5)