FastQCFastQC Report
Thu 26 May 2016
SRR1511871_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511871_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences153070
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT17081.1158293591167439No Hit
GGTATCAACGCAGAGTACTTTTTTT11420.7460638923368393No Hit
TATCAACGCAGAGTACTTTTTTTTT10590.6918403344874894No Hit
ACGCAGAGTACTTTTTTTTTTTTTT5710.37303194616841967No Hit
GTACTTTTTTTTTTTTTTTTTTTTT4440.2900633697001372No Hit
GAGTACTTTTTTTTTTTTTTTTTTT4180.2730776768798589No Hit
GGTATCAACGCAGAGTACATGGGGG2510.16397726530345594No Hit
GCAGAGTACTTTTTTTTTTTTTTTT2200.14372509309466258No Hit
GTGGTATCAACGCAGAGTACTTTTT1600.10452734043248187No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1800.016.9721131
GTGGTAT350.00208840516.3659671
ATAATGG500.001485127813.3043493
AATGGAG657.9292984E-411.6961325
GTATCAA4600.011.4146681
AGGCTGA900.00110809169.493793510
GGAGGCT900.00110809169.49379358
TATCAAC6350.08.2310382
GAGGCTG1050.00449121658.1375379
ATCAACG6800.07.82608843
TCAACGC6950.07.657184
CAACGCA6900.07.574945
AACGCAG7100.07.3591556
CGCAGAG8050.06.48644268
ACGCAGA8100.06.4464037
TACATGG3502.8287104E-86.2448992
AGAGTAC8200.06.13623211
GTACATG3604.58258E-86.09935331
GCAGAGT8250.06.0990439
CAGAGTA8300.06.06230210
Produced by FastQC (version 0.11.5)