##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511871_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 153070 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.631815509244138 31.0 30.0 33.0 26.0 34.0 2 29.79997386816489 31.0 30.0 34.0 26.0 34.0 3 29.81971646958908 31.0 30.0 34.0 26.0 34.0 4 33.440243026066504 35.0 33.0 37.0 28.0 37.0 5 33.003318743058735 35.0 33.0 37.0 27.0 37.0 6 33.041739073626445 35.0 33.0 37.0 27.0 37.0 7 32.83192003658457 35.0 33.0 37.0 26.0 37.0 8 32.69837329326452 35.0 33.0 37.0 26.0 37.0 9 33.857757888547724 37.0 33.0 39.0 26.0 39.0 10 33.55492258443849 37.0 33.0 39.0 25.0 39.0 11 33.67374403867512 37.0 33.0 39.0 25.0 39.0 12 33.30552688312537 37.0 33.0 39.0 23.0 39.0 13 33.330208401384986 37.0 33.0 39.0 23.0 39.0 14 33.908407917946036 38.0 33.0 40.0 22.0 41.0 15 33.76274906905338 38.0 33.0 40.0 19.0 41.0 16 33.61100803553929 38.0 32.0 40.0 18.0 41.0 17 33.52632782387143 38.0 32.0 40.0 18.0 41.0 18 33.64082445939766 38.0 33.0 40.0 18.0 41.0 19 33.739459071013265 38.0 33.0 40.0 18.0 41.0 20 33.57122231658718 38.0 33.0 40.0 18.0 41.0 21 33.421571829881756 38.0 32.0 40.0 18.0 41.0 22 33.23463774743581 38.0 32.0 40.0 15.0 41.0 23 32.97537727836937 38.0 32.0 40.0 10.0 41.0 24 32.63855098974325 38.0 32.0 40.0 2.0 41.0 25 31.695165610504997 37.0 31.0 39.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.8837884531360096 1101 2 -0.8550167546638612 1101 3 -0.8828608456077589 1101 4 -0.8194735335114132 1101 5 -1.0474885747332294 1101 6 -1.248443129382835 1101 7 -0.9408441056990746 1101 8 -1.0836037095356055 1101 9 -0.6913055221306337 1101 10 -1.0910953186698436 1101 11 -1.1477921103421735 1101 12 -1.1825432965520122 1101 13 -1.3254652494240133 1101 14 -1.3004919537742836 1101 15 -1.9195055333758084 1101 16 -0.724744695629937 1101 17 -1.621694897778415 1101 18 -1.801353267036582 1101 19 -1.424309006464675 1101 20 -2.2323217125268826 1101 21 -1.8312213992253241 1101 22 -1.5818689381950186 1101 23 -1.7568519937858191 1101 24 -1.3903595162481786 1101 25 -1.089321483256576 1102 1 0.01972340279586149 1102 2 -0.20253032941287685 1102 3 -0.2543597322830209 1102 4 0.14181401678352046 1102 5 0.03931169090214581 1102 6 -0.14082173792223074 1102 7 -0.039129194960374036 1102 8 -0.6199918496973282 1102 9 0.24423883507081712 1102 10 0.28782573293374014 1102 11 -0.2730704415269827 1102 12 0.06418509127563254 1102 13 0.3319041790692907 1102 14 0.5921601029466856 1102 15 0.5415281007505257 1102 16 0.28861520124102924 1102 17 -0.4033660568080606 1102 18 -0.5509528607707139 1102 19 -0.11859393946986785 1102 20 0.03612627372258004 1102 21 0.28487688586393745 1102 22 0.35969127900096254 1102 23 0.4874544244336434 1102 24 1.3507631838572962 1102 25 0.572423116141838 1103 1 -0.14753795132950742 1103 2 -0.042625479406268596 1103 3 0.06963480540254352 1103 4 -0.47128672767707513 1103 5 0.18106756068761598 1103 6 0.29805760660003955 1103 7 0.10465301727697351 1103 8 0.37190278049516934 1103 9 0.08455423967854614 1103 10 -0.13404995536193098 1103 11 0.04744407325210176 1103 12 0.11171603704895006 1103 13 0.14596523364838276 1103 14 -0.1902283380911527 1103 15 0.23625520528336352 1103 16 -0.2166488840415539 1103 17 0.666542757781599 1103 18 0.3204184444581344 1103 19 0.816959560375949 1103 20 0.6259681309094844 1103 21 0.39412317457368573 1103 22 0.8555725177129148 1103 23 1.1438572260723348 1103 24 1.433138409618273 1103 25 0.25913295271291403 1104 1 0.9426659493797125 1104 2 0.6138904780405419 1104 3 0.91147877703375 1104 4 0.9516742652307286 1104 5 0.7149328089145683 1104 6 0.41507888319578257 1104 7 0.1409562087663332 1104 8 0.3059010074455273 1104 9 1.41376523258635 1104 10 1.1682993354182116 1104 11 0.8923022292804674 1104 12 1.135341923573769 1104 13 0.9740237442866828 1104 14 1.4017486122634537 1104 15 0.7562020137940024 1104 16 1.400727002483265 1104 17 1.7350268003347935 1104 18 1.6121294373659367 1104 19 1.4607538866170842 1104 20 1.315241180554878 1104 21 1.0771905504602088 1104 22 1.7387285460817097 1104 23 1.2973146728808516 1104 24 1.270017842242389 1104 25 2.006118768315755 1105 1 -0.7728789224151598 1105 2 -0.9131928552927917 1105 3 -0.8013417357867638 1105 4 -1.3845436834872231 1105 5 -1.8036569346751747 1105 6 -1.7579980398811408 1105 7 -1.910486098925972 1105 8 -0.7051566848621675 1105 9 -0.6363950238239084 1105 10 -0.8507711774023008 1105 11 -0.5339798220015837 1105 12 -0.8401388456569947 1105 13 -1.0097903582774208 1105 14 -1.236873182608342 1105 15 -0.9409133708213844 1105 16 -1.1300422282859657 1105 17 -1.2321206355626444 1105 18 -0.9966846651981669 1105 19 -1.0065538056906078 1105 20 -0.9363613835476841 1105 21 -0.7032581674885066 1105 22 -0.7734509410977779 1105 23 -0.5453135322473557 1105 24 -0.5056231833986402 1105 25 -1.0624709752739925 1106 1 0.26558261604638034 1106 2 0.22783278573284704 1106 3 0.2819915975465719 1106 4 0.7372511883076456 1106 5 0.42070203968380326 1106 6 0.5048224729393738 1106 7 -0.08997327841315439 1106 8 0.42304844334296376 1106 9 0.17129728386839815 1106 10 0.3735877969566701 1106 11 0.6390971010753361 1106 12 0.1470406415224872 1106 13 0.09918400069693689 1106 14 0.7374857917506361 1106 15 1.251394321486309 1106 16 1.8699577717140343 1106 17 1.1204434670014578 1106 18 0.689212770699271 1106 19 0.8524205532837499 1106 20 0.9995360523497467 1106 21 1.1236649094345665 1106 22 0.9188567512099155 1106 23 0.7303274933936663 1106 24 1.1995050217295713 1106 25 -0.29324020604322243 1107 1 0.5178348682986318 1107 2 0.3255458834459475 1107 3 0.5425598581148314 1107 4 0.5709310219874837 1107 5 0.2237165926983522 1107 6 0.698862666979565 1107 7 0.5999089114690364 1107 8 0.40606992636444517 1107 9 0.9713665839377015 1107 10 0.4188060921749681 1107 11 0.415950877929113 1107 12 0.1858486803305297 1107 13 0.004935906448842786 1107 14 0.3012418555066958 1107 15 0.4437020137940024 1107 16 0.3304567322129941 1107 17 0.4335065300645198 1107 18 -0.2319584004719033 1107 19 -0.17235422149101964 1107 20 0.14801145082515177 1107 21 0.4673932531629106 1107 22 0.6056204379736059 1107 23 -0.09018532711915128 1107 24 -0.28403621181166727 1107 25 -0.7107731235761392 1108 1 0.9164835169472809 1108 2 0.7985188564189194 1108 3 1.13039769595267 1108 4 1.3141742652307258 1108 5 1.054797673779433 1108 6 0.6785923967092984 1108 7 0.4647737763338995 1108 8 1.669583439877961 1108 9 0.9781233406944523 1108 10 1.4390763624452347 1108 11 1.1794643914426288 1108 12 0.9290919235737718 1108 13 1.1535845550974955 1108 14 1.7607013149661555 1108 15 1.3626209327129217 1108 16 1.5061324078886713 1108 17 1.365938962496955 1108 18 1.74777132925783 1108 19 1.3614295622927628 1108 20 1.749362802176499 1108 21 1.0484743342439913 1108 22 1.4164312487844128 1108 23 1.6125173755835505 1108 24 1.3646124368369854 1108 25 1.0459836331806152 1109 1 0.06809403157149418 1109 2 -0.13807870004164968 1109 3 0.04024590032142328 1109 4 0.2833523474225075 1109 5 -0.7381493828662542 1109 6 0.3123391571683882 1109 7 -0.14046502411037665 1109 8 -0.112164061047622 1109 9 -0.2635464112492727 1109 10 0.00793974637711159 1109 11 0.18511044845855196 1109 12 0.6585439783682929 1109 13 0.4078422374373645 1109 14 0.5237520369209889 1109 15 0.20055132886249538 1109 16 0.37812426275723965 1109 17 0.2551466633484907 1109 18 0.3901260127084001 1109 19 0.7268497770280433 1109 20 0.884590495623371 1109 21 0.01469055046020884 1109 22 -0.6766824128224016 1109 23 -0.7408702586260034 1109 24 -0.23683147282610406 1109 25 -0.3599771220952057 1110 1 1.0660212125376063 1110 2 1.1701404780405404 1110 3 0.8759524612442782 1110 4 1.1299637389149382 1110 5 0.8083538615461521 1110 6 0.9084999358273649 1110 7 0.5814167350821222 1110 8 1.3029404811297383 1110 9 0.8502783904810869 1110 10 1.4646809143655801 1110 11 1.0699338082278373 1110 12 1.3172498183106143 1110 13 1.2835632179708938 1110 14 1.873788085947666 1110 15 1.3976493822150573 1110 16 1.6599375287990554 1110 17 1.4077241687558484 1110 18 1.0861425952606751 1110 19 0.6857538866170856 1110 20 1.877741180554878 1110 21 1.2910063399338938 1110 22 1.4571495987132934 1110 23 1.5545515149861089 1110 24 1.645017842242389 1110 25 0.5166450841052246 1111 1 -0.6883511313656285 1111 2 -0.3578098325184662 1111 3 -0.39721687514016324 1111 4 -0.4917263558872875 1111 5 -0.029011290464310946 1111 6 -0.22777825966136334 1111 7 -0.281365219805096 1111 8 -0.4833458869644147 1111 9 -1.612904022072037 1111 10 -2.060000354022783 1111 11 -1.4159275843841286 1111 12 -0.8923366478547976 1111 13 -1.450704516582885 1111 14 -1.5755417604073543 1111 15 -1.5392172408643816 1111 16 -1.3325028111813282 1111 17 -1.5462231996652065 1111 18 -2.3149280160502137 1111 19 -2.13722747984254 1111 20 -1.2930662728612745 1111 21 -1.4977318097882382 1111 22 -1.652731081247481 1111 23 -1.8603716625228763 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2315 2 -0.34558279239971057 2315 3 -0.3671375751675079 2315 4 -0.5867691309956911 2315 5 -0.5380546124690753 2315 6 -0.5440406136595612 2315 7 -0.7773220931204605 2315 8 -0.42322635104503803 2315 9 0.23951208793226186 2315 10 -0.2797745639528557 2315 11 0.009440594060343699 2315 12 -0.48443008900484585 2315 13 -0.5267231110592334 2315 14 -0.1621271739000676 2315 15 -0.44780742016826025 2315 16 -0.831662934623651 2315 17 -0.6971665958916233 2315 18 -1.109529367665509 2315 19 -0.4934913964017795 2315 20 -1.3140827188161879 2315 21 -1.051347185388849 2315 22 -1.317717994798791 2315 23 -1.4801224340373906 2315 24 -1.9563972520972328 2315 25 -1.8440384643886496 2316 1 0.1390394061698359 2316 2 -0.05825458368785519 2316 3 -0.010743445188470702 2316 4 0.22466809239121943 2316 5 -0.7822894133076517 2316 6 -0.4799828451992809 2316 7 -0.37131230975218443 2316 8 0.18776829139614648 2316 9 -0.39233044642600134 2316 10 -0.050913627544751705 2316 11 -0.4178829559047159 2316 12 -0.2036241258089433 2316 13 -0.29611514460220434 2316 14 -0.7197791655143249 2316 15 -0.5100016899097 2316 16 0.116159101248698 2316 17 0.18217186206318559 2316 18 0.2139041287239607 2316 19 -0.48708561955575647 2316 20 -0.31361684413648305 2316 21 0.21222141465774058 2316 22 -0.22454305885656112 2316 23 -0.8679631048969298 2316 24 -0.16208092318971268 2316 25 0.094699015229331 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4383.0 3 1.0 4 65.0 5 494.0 6 660.0 7 569.0 8 508.0 9 458.0 10 434.0 11 392.0 12 413.0 13 400.0 14 380.0 15 405.0 16 363.0 17 438.0 18 407.0 19 482.0 20 559.0 21 742.0 22 933.0 23 1175.0 24 1394.0 25 1682.0 26 1880.0 27 2205.0 28 2630.0 29 3214.0 30 4067.0 31 5192.0 32 6850.0 33 8909.0 34 11831.0 35 15782.0 36 20893.0 37 27904.0 38 23976.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.49084969892557 22.21653744736117 14.449604470856784 20.843008382856468 2 17.569086039067095 24.24511661331417 36.88900503037826 21.296792317240477 3 19.807931011955315 27.2881688116548 31.74429999346704 21.159600182922848 4 15.257071927876135 19.700137192134317 36.65316521852747 28.389625661462077 5 13.391672602981448 39.322149098442615 34.58921790958938 12.696960388986556 6 30.844750052290316 39.34454089102698 16.955134909014852 12.855574147667854 7 26.659698177304502 34.82720324034755 22.003658456915137 16.50944012543281 8 26.269680538315804 36.483308290324686 20.132619063173713 17.114392108185797 9 27.35278937430993 15.57789929636816 20.70193319090832 36.36737813841359 10 15.868556869406152 30.146338276605473 33.6016201737767 20.383484680211666 11 34.41432024563925 23.951133468347816 23.761024367936237 17.873521918076698 12 22.75233553276279 27.330633043705493 30.785914940876722 19.131116482654996 13 29.107597831057685 22.9287254197426 26.310838178611096 21.65283857058862 14 22.05461553537597 22.814398641144574 28.473900829685768 26.65708499379369 15 23.988371333376886 30.08427516822369 24.87424054354217 21.053112954857255 16 22.24276474815444 29.945776442150652 26.598941660678122 21.21251714901679 17 21.53589860847978 29.012216632913045 28.133533677402493 21.31835108120468 18 21.528712353825046 28.0263931534592 29.809890899588424 20.635003593127326 19 22.698733151701653 27.874582671815073 30.362544672899638 19.064139503583633 20 22.777814071993205 26.982426340889788 30.262624942836613 19.977134644280394 21 23.448402017404028 26.967099589724832 29.854705098387647 19.729793294483496 22 23.45378167730471 26.6595673789909 29.982948643404523 19.90370230029987 23 23.05199472081749 26.981980216132868 29.76465822520156 20.20136683784808 24 22.747922944679406 27.41843758375223 29.755982769102047 20.07765670246632 25 22.280527492060173 27.371165684262806 30.058944231699186 20.289362591977834 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 95.0 1 95.0 2 130.5 3 166.0 4 166.0 5 166.0 6 395.5 7 625.0 8 625.0 9 625.0 10 755.0 11 885.0 12 885.0 13 885.0 14 1014.0 15 1143.0 16 1143.0 17 1143.0 18 1977.0 19 2811.0 20 2811.0 21 2811.0 22 4086.0 23 5361.0 24 5361.0 25 5361.0 26 7134.0 27 8907.0 28 8907.0 29 8907.0 30 10696.5 31 12486.0 32 12486.0 33 12486.0 34 13715.0 35 14944.0 36 14944.0 37 14944.0 38 15650.0 39 16356.0 40 16356.0 41 16356.0 42 16989.0 43 17622.0 44 17622.0 45 17622.0 46 18091.0 47 18560.0 48 18560.0 49 18560.0 50 18185.5 51 17811.0 52 17811.0 53 17811.0 54 15969.0 55 14127.0 56 14127.0 57 14127.0 58 12036.0 59 9945.0 60 9945.0 61 9945.0 62 7933.5 63 5922.0 64 5922.0 65 5922.0 66 4504.5 67 3087.0 68 3087.0 69 3087.0 70 2232.0 71 1377.0 72 1377.0 73 1377.0 74 979.5 75 582.0 76 582.0 77 582.0 78 378.5 79 175.0 80 175.0 81 175.0 82 123.5 83 72.0 84 72.0 85 72.0 86 41.0 87 10.0 88 10.0 89 10.0 90 5.5 91 1.0 92 1.0 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4024302606650552 2 0.0 3 0.0 4 0.0 5 0.037237865029071664 6 0.05095707846083491 7 0.0 8 0.0 9 0.004573071143921081 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.008492846410139152 20 0.0 21 0.0013065917554060233 22 0.001959887633109035 23 0.010452734043248186 24 0.05814333311556804 25 0.028745018618932517 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 153070.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.20796878051054 #Duplication Level Percentage of deduplicated Percentage of total 1 77.96555301082546 52.39906452706797 2 13.789920940144205 18.535851520618408 3 4.2965362648962255 8.662844254664307 4 1.8320016262282874 4.925004324055812 5 0.8409954607955149 2.826079833684802 6 0.38799778215081154 1.5645925697819443 7 0.27599841789445867 1.2984505137324756 8 0.15799909417245148 0.849503855079285 9 0.11299935215661715 0.6835011238763857 >10 0.31599818833108484 3.364411628434263 >50 0.009999942668705008 0.4361772167107087 >100 0.009999942668705008 1.4436188929038556 >500 9.999942668705007E-4 0.3837553016052614 >1k 0.0029999828006115024 2.6271444377845303 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1708 1.1158293591167439 No Hit GGTATCAACGCAGAGTACTTTTTTT 1142 0.7460638923368393 No Hit TATCAACGCAGAGTACTTTTTTTTT 1059 0.6918403344874894 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 571 0.37303194616841967 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 444 0.2900633697001372 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 418 0.2730776768798589 No Hit GGTATCAACGCAGAGTACATGGGGG 251 0.16397726530345594 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 220 0.14372509309466258 No Hit GTGGTATCAACGCAGAGTACTTTTT 160 0.10452734043248187 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 180 0.0 16.972113 1 GTGGTAT 35 0.002088405 16.365967 1 ATAATGG 50 0.0014851278 13.304349 3 AATGGAG 65 7.9292984E-4 11.696132 5 GTATCAA 460 0.0 11.414668 1 AGGCTGA 90 0.0011080916 9.4937935 10 GGAGGCT 90 0.0011080916 9.4937935 8 TATCAAC 635 0.0 8.231038 2 GAGGCTG 105 0.0044912165 8.137537 9 ATCAACG 680 0.0 7.8260884 3 TCAACGC 695 0.0 7.65718 4 CAACGCA 690 0.0 7.57494 5 AACGCAG 710 0.0 7.359155 6 CGCAGAG 805 0.0 6.4864426 8 ACGCAGA 810 0.0 6.446403 7 TACATGG 350 2.8287104E-8 6.244899 2 AGAGTAC 820 0.0 6.136232 11 GTACATG 360 4.58258E-8 6.0993533 1 GCAGAGT 825 0.0 6.099043 9 CAGAGTA 830 0.0 6.062302 10 >>END_MODULE