FastQCFastQC Report
Thu 26 May 2016
SRR1511870_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511870_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences780131
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT29140.37352701020726004No Hit
GGTATCAACGCAGAGTACTTTTTTT18450.23649874187796666No Hit
TATCAACGCAGAGTACTTTTTTTTT16480.21124657269099675No Hit
GTCCTACAGTGGACATTTCTAAATT16370.20983655309172433No Hit
CTGTAGGACGTGGAATATGGCAAGA14100.18073887590673873No Hit
GTCCTAAAGTGTGTATTTCTCATTT11360.14561656952486185No Hit
GTCCTACAGTGTGCATTTCTCATTT9880.12664539673465097No Hit
CTTTAGGACGTGAAATATGGCGAGG9800.12561992793518012No Hit
ACGCAGAGTACTTTTTTTTTTTTTT9120.11690344313967783No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTAGG504.5142788E-617.116691
TTAGTCC500.001499675313.2984754
GTGGTAT1650.013.2553481
GGTATCA6000.012.9960041
CTAGGGT604.091925E-412.6652144
TAGGACC3150.012.0621094
TAGAAAT2300.011.9768884
TGGTATC1701.8189894E-1211.7467472
ATTTAGA1800.011.6224431
CCTATTC752.0735002E-411.3986923
GTCCTAC8200.011.1328071
GCTTAGG600.00582872911.0941511
TTAGTCT1201.2734199E-711.0820624
TCCTACA8850.011.0672882
GACGTGG3850.010.8558987
GTGTAGA1355.5862984E-810.5658571
TAATACA1355.6765202E-810.5543454
ATAGCCC909.511279E-510.5543453
TGTAGAA1909.094947E-1210.5102482
TGTAGGA7700.010.4972492
Produced by FastQC (version 0.11.5)