FastQCFastQC Report
Thu 26 May 2016
SRR1511870_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511870_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences780131
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT36540.4683828741583144No Hit
GGTATCAACGCAGAGTACTTTTTTT23600.30251329584390313No Hit
TATCAACGCAGAGTACTTTTTTTTT21430.2746974546582561No Hit
GTCCTACAGTGGACATTTCTAAATT17940.22996137828133995No Hit
CTGTAGGACGTGGAATATGGCAAGA14300.1833025479054159No Hit
GTCCTAAAGTGTGTATTTCTCATTT11980.15356395272076098No Hit
ACGCAGAGTACTTTTTTTTTTTTTT11590.14856479232334055No Hit
CTTTAGGACGTGAAATATGGCGAGG9670.12395354113603997No Hit
GTCCTACAGTGTGCATTTCTCATTT9520.12203078713703211No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAC400.00528035614.248219518
AACCATC400.00529610214.2418287
GTTTTAG701.04595085E-412.2678261
TAGGACC3300.012.0956044
GGTATCA5700.011.8852031
GTGGTAT1700.011.7867351
TGTAGGA7850.011.6223462
GTAGGAC7750.011.4044283
CTGTAGG7950.011.2819421
ATAGCAC600.005854262511.0876383
TATACTG1803.6379788E-1211.0876375
AGGACGT6900.011.0187695
AATACTG951.3518458E-511.00427155
GGACGTG6750.010.8370736
GTCCTAC8800.010.7343481
GGCGAGG3400.010.61632219
TAGGACG7550.010.5736414
TACTATA1002.3839286E-510.4540582
GTCCTAA3400.010.3835531
GTATCAA11300.010.3015971
Produced by FastQC (version 0.11.5)