FastQCFastQC Report
Thu 26 May 2016
SRR1511869_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511869_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences196795
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT47942.436037500952768No Hit
GGTATCAACGCAGAGTACTTTTTTT30401.5447546939708834No Hit
TATCAACGCAGAGTACTTTTTTTTT29611.504611397647298No Hit
ACGCAGAGTACTTTTTTTTTTTTTT14730.7484946263878656No Hit
GTACTTTTTTTTTTTTTTTTTTTTT14120.7174979039101603No Hit
GAGTACTTTTTTTTTTTTTTTTTTT10320.5244035671637999No Hit
GTACTGGTTCACTATCGGTCAGTCA6350.322670799562997No Hit
GCAGAGTACTTTTTTTTTTTTTTTT6050.3074265098198633No Hit
GTGGTATCAACGCAGAGTACATGGG5720.29065779110241624No Hit
ATCAACGCAGAGTACTTTTTTTTTT4050.2057979115323052No Hit
GGTATCAACGCAGAGTACATGGGGG3850.1956350517035494No Hit
ATCATTAACTGAATCCATAGGTTAA3790.1925861937549226No Hit
GGATACCACGTGTCCCGCCCTACTC3770.19156990777204705No Hit
GGTTAATGAGGCGAACCGGGGGAAC3520.17886633298610227No Hit
GTGGTATCAACGCAGAGTACTTTTT3520.17886633298610227No Hit
ATACAGGGTGACAGCCCCGTACACA3400.1727686170888488No Hit
GTCCTACAGTGGACATTTCTAAATT3290.16717904418303312No Hit
CTGTAGGACGTGGAATATGGCAAGA3120.15854061332859068No Hit
GTATCAACGCAGAGTACATGGGGGT2800.14228003760258137No Hit
GTACATGGGGGTTAAGCGACTAAGC2730.13872303666251684No Hit
AGCGTACACGGTGGATGCCCTGGCA2700.13719860768820347No Hit
GGTATCAACGCAGAGTACATGGGGA2680.13618232170532787No Hit
GTCCTAAAGTGTGTATTTCTCATTT2640.13414974973957672No Hit
GTATTTAGCCTTGGAGGATGGTCCC2560.1300846058080744No Hit
GTGGATGCCCTGGCAGTCAGAGGCG2550.1295764628166366No Hit
CCCATATTCAGACAGGATACCACGT2330.1183973170050052No Hit
GTTCACTATCGGTCAGTCAGGAGTA2310.11738103102212963No Hit
TATTCAGACAGGATACCACGTGTCC2300.11687288803069185No Hit
GTTAATGATAGTGTGTCGAAACACA2300.11687288803069185No Hit
CTTTAGGACGTGAAATATGGCGAGG2290.11636474503925404No Hit
GTCAGGAGTATTTAGCCTTGGAGGA2250.11433217307350288No Hit
TATCAACGCAGAGTACATGGGGGTT2240.1138240300820651No Hit
CTATCGGTCAGTCAGGAGTATTTAG2240.1138240300820651No Hit
ACTTAGATGTTTCAGTTCCCCCGGT2110.1072181711933738No Hit
TCTAAGTACCCCGAGGAAAAGAAAT2110.1072181711933738No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCATCCC250.006017498718.9979694
ACAGTCC350.002162698216.2839728
GGATCAA601.4372654E-615.8477491
GGGGAAC851.0004442E-915.64538519
ACCGGGG807.588824E-915.43584915
GAACCGG807.588824E-915.43584913
GCGACTA653.3398119E-614.61382116
CGACTAA653.3398119E-614.61382117
AACCGGG851.6887498E-814.52785814
CCGGGGG851.6887498E-814.52785816
TAGACCA400.00526118314.2484764
GACCAGG400.00526118314.2484766
CTGGCCT400.00526118314.24847613
GGTATCA6300.014.0365771
AAGGACC551.9482622E-413.8167044
AGGACCT903.580317E-813.7207555
CGGGGGA903.580317E-813.72075517
GATCAAC707.118435E-613.5837852
AAGCGAC707.1937047E-613.56997714
AGCGACT707.1937047E-613.56997715
Produced by FastQC (version 0.11.5)