FastQCFastQC Report
Thu 26 May 2016
SRR1511869_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511869_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences196795
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT56282.8598287558118853No Hit
GGTATCAACGCAGAGTACTTTTTTT37551.9080769328489036No Hit
TATCAACGCAGAGTACTTTTTTTTT36581.858787062679438No Hit
ACGCAGAGTACTTTTTTTTTTTTTT18420.9359993902284102No Hit
GTACTTTTTTTTTTTTTTTTTTTTT16420.8343707919408522No Hit
GAGTACTTTTTTTTTTTTTTTTTTT13630.6925988973297086No Hit
GCAGAGTACTTTTTTTTTTTTTTTT7550.3836479585355319No Hit
GTGGTATCAACGCAGAGTACATGGG6100.3099672247770523No Hit
GTACTGGTTCACTATCGGTCAGTCA5480.27846235930790925No Hit
ATCAACGCAGAGTACTTTTTTTTTT5230.26575878452196444No Hit
ATCATTAACTGAATCCATAGGTTAA4930.25051449477883075No Hit
GTGGTATCAACGCAGAGTACTTTTT4450.22612363118981682No Hit
GGTATCAACGCAGAGTACATGGGGG3980.20224091059224064No Hit
GGTTAATGAGGCGAACCGGGGGAAC3650.18547219187479358No Hit
GGATACCACGTGTCCCGCCCTACTC3430.17429304606316218No Hit
ATACAGGGTGACAGCCCCGTACACA3200.162605757260093No Hit
CTGTAGGACGTGGAATATGGCAAGA3150.16006504230290403No Hit
GTCCTACAGTGGACATTTCTAAATT3080.1565080413628395No Hit
GTATCAACGCAGAGTACATGGGGGT3060.15549175537996393No Hit
AGCGTACACGGTGGATGCCCTGGCA2950.14990218247414822No Hit
GGTATCAACGCAGAGTACATGGGGA2940.14939403948271043No Hit
GTACATGGGGGTTAAGCGACTAAGC2620.13313346375670113No Hit
GTGGATGCCCTGGCAGTCAGAGGCG2590.13160903478238775No Hit
GTCCTAAAGTGTGTATTTCTCATTT2510.12754389085088544No Hit
GTTAATGATAGTGTGTCGAAACACA2370.12042988897075636No Hit
GTATTTAGCCTTGGAGGATGGTCCC2290.11636474503925404No Hit
ACTTAGATGTTTCAGTTCCCCCGGT2230.11331588709062732No Hit
CCCATATTCAGACAGGATACCACGT2190.11128331512487614No Hit
GTTCACTATCGGTCAGTCAGGAGTA2140.10874260016768718No Hit
TATCAACGCAGAGTACATGGGGGTT2070.10518559922762265No Hit
TATTCAGACAGGATACCACGTGTCC2030.10315302726187148No Hit
CTTTAGGACGTGAAATATGGCGAGG2000.10162859828755813No Hit
TCTAAGTACCCCGAGGAAAAGAAAT2000.10162859828755813No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATTGT307.5279735E-419.0602041
CACGGCA250.006036594518.98754911
ACGGCAA250.006036594518.98754912
TAGGACC402.7275746E-416.6394754
AGGACCT807.490598E-915.4509425
GGTATCA7050.014.8696621
TAATAGA400.00522714114.2624074
AAGGACC400.00522714114.2624074
CGCGCCT400.005280366614.24066112
CGCCTTT551.9573502E-413.80912614
GGACCTG957.224662E-813.00476
GTGGTAT1800.012.7068021
CCGGGGG604.0864095E-412.65836616
ACCGGGG604.0864095E-412.65836615
TGGTATC1750.012.4965862
GACCTGG1001.4298348E-712.3419077
GAATCCA853.922776E-612.2860611
TGAATCC853.922776E-612.2860610
AGGGTGA701.07282394E-412.2249215
CCCCGGT550.003049640512.09220419
Produced by FastQC (version 0.11.5)