FastQCFastQC Report
Thu 26 May 2016
SRR1511868_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511868_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences868613
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT15510.17856053271134556No Hit
GTCCTAAAGTGTGTATTTCTCATTT15300.1761428852665111No Hit
GTCCTACAGTGGACATTTCTAAATT15110.1739554899592799No Hit
CTGTAGGACGTGGAATATGGCAAGA14730.16958069934481756No Hit
CTTTAGGACGTGAAATATGGCGAGG12500.14390758600205156No Hit
TATCAACGCAGAGTACTTTTTTTTT9390.10810337860474113No Hit
GGTATCAACGCAGAGTACTTTTTTT8930.10280757943986563No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG500.001499851213.2985518
GGTATCA3550.012.8569911
TAGGACC2200.012.0895914
CCTATAC658.024513E-411.6910323
ATTTAGA2800.011.206821
GCCTTAA600.00583171111.0936191
TTAGTAG600.005876892211.0821253
AATGTCC2500.011.0187988
CTAAGAC951.3598135E-510.99883
AACTGAT951.3598135E-510.99887
GTCCTAA5450.010.9918431
GTCCTAC9100.010.8672191
AAATGTC2800.010.8559597
GGCGAGG3200.010.68633519
AGGACGT8200.010.6573735
CCTACAG9250.010.5772243
TAGAAAT3600.010.5544044
TCCTACA9700.010.4891072
TAGACTG1553.6543497E-910.4182195
GTATTAG1105.967684E-610.3732551
Produced by FastQC (version 0.11.5)