FastQCFastQC Report
Thu 26 May 2016
SRR1511868_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511868_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences868613
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT19220.22127230423675445No Hit
CTGTAGGACGTGGAATATGGCAAGA15600.17959666733056032No Hit
GTCCTACAGTGGACATTTCTAAATT15360.17683364167932095No Hit
GTCCTAAAGTGTGTATTTCTCATTT14710.16935044720721426No Hit
CTTTAGGACGTGAAATATGGCGAGG12830.1477067462725057No Hit
TATCAACGCAGAGTACTTTTTTTTT11950.13757565221796128No Hit
GGTATCAACGCAGAGTACTTTTTTT11820.13607901332353994No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGAGG4200.013.11988319
GGTATCA4150.013.1018511
CTAGGAC701.0862583E-412.2185823
TTAGAAC1103.7862264E-812.0951613
TAGAACC657.993542E-411.696424
TAGGACC3050.011.5286454
TGGCGAG8650.011.20302518
GACGTGA4850.011.1592317
TACACTC600.00585676311.0872335
GGACGTG8300.010.9867536
AGGACGT8650.010.766795
GTCCTAA5000.010.6837541
GTGTAGG1355.3390977E-810.5989631
TTAGGAC5850.010.5592683
ACGTGAA5500.010.358338
ATGGCGA9400.010.30382417
AATGTCC2500.010.2547468
TGTAGGA9550.010.2496782
GTATTGA750.002556980110.1750051
TATGGCG9250.010.16294716
Produced by FastQC (version 0.11.5)