FastQCFastQC Report
Thu 26 May 2016
SRR1511867_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511867_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences989525
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT39830.4025163588590485No Hit
GGTATCAACGCAGAGTACTTTTTTT28220.2851873373588338No Hit
TATCAACGCAGAGTACTTTTTTTTT27790.28084181804401104No Hit
GTCCTAAAGTGTGTATTTCTCATTT14940.150981531542912No Hit
GTCCTACAGTGGACATTTCTAAATT14690.1484550668249918No Hit
CTGTAGGACGTGGAATATGGCAAGA13140.13279098557388647No Hit
CTTTAGGACGTGAAATATGGCGAGG12620.1275359389606124No Hit
ACGCAGAGTACTTTTTTTTTTTTTT12160.12288724387963923No Hit
GTACTTTTTTTTTTTTTTTTTTTTT11490.11611631843561304No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA250.006235259618.89049319
GATAGCG250.006237079418.8895427
CTTCGTG400.00548540114.1671559
TAGGACC2850.013.9186094
GGTATCA7550.013.5042981
CTAGGAC1155.7934813E-912.3192663
GGATAAC655.228709E-412.3025591
GTCTTAG1153.4804543E-812.1688361
CTTAGAC803.0399277E-511.8059633
AGGCGAA658.4207713E-411.6243329
GTCCTAT1251.10740075E-711.1953281
TGGTATC2500.011.1953282
GACGTGG4050.011.1938027
TAGACTG1355.167749E-911.1938015
AATCCCG700.00156418710.79456819
CTCCGGG650.00729479610.7647392
TAGCACC803.9765862E-410.6253664
GTATAAG1602.6630005E-910.6205681
ACGAAAT901.0084834E-410.49418912
GTAGGAC9500.010.4389563
Produced by FastQC (version 0.11.5)