##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511867_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 989525 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.926988201409767 31.0 30.0 34.0 25.0 34.0 2 27.1112109345393 31.0 27.0 34.0 10.0 34.0 3 28.89301129329729 31.0 28.0 34.0 16.0 34.0 4 33.351937545792175 35.0 33.0 37.0 28.0 37.0 5 33.7404603218716 35.0 35.0 37.0 30.0 37.0 6 33.957964680023245 37.0 35.0 37.0 30.0 37.0 7 33.95982415805563 37.0 35.0 37.0 30.0 37.0 8 33.976945504156035 37.0 35.0 37.0 30.0 37.0 9 35.57498395694904 39.0 35.0 39.0 30.0 39.0 10 35.45486875015791 39.0 35.0 39.0 30.0 39.0 11 35.466561228872436 39.0 35.0 39.0 30.0 39.0 12 35.36644955913191 39.0 35.0 39.0 30.0 39.0 13 35.360856976832316 39.0 35.0 39.0 30.0 39.0 14 36.491843056011724 40.0 37.0 41.0 30.0 41.0 15 36.43860235971805 40.0 36.0 41.0 29.0 41.0 16 36.39403653267982 40.0 36.0 41.0 29.0 41.0 17 36.36277405826028 40.0 36.0 41.0 29.0 41.0 18 36.338387610217026 40.0 36.0 41.0 29.0 41.0 19 36.36655567065006 40.0 37.0 41.0 29.0 41.0 20 36.31413557009677 40.0 37.0 41.0 29.0 41.0 21 36.21167529875445 40.0 37.0 41.0 29.0 41.0 22 36.12368863848816 40.0 36.0 41.0 29.0 41.0 23 36.028060938329 40.0 36.0 41.0 28.0 41.0 24 35.93002905434425 40.0 36.0 41.0 28.0 41.0 25 35.831800106111515 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.0185074338816946 1101 2 3.5568608873615766 1101 3 2.2647465491477057 1101 4 1.1970923697688463 1101 5 0.6273265334983691 1101 6 0.6571022091993868 1101 7 0.4649475974293935 1101 8 0.5457837001014454 1101 9 0.7150444544091883 1101 10 0.551672899446352 1101 11 0.6382371284241799 1101 12 0.4518338121070542 1101 13 0.507242845249344 1101 14 0.66625031000288 1101 15 0.6267015775597358 1101 16 0.6044724435033046 1101 17 0.567282526157463 1101 18 0.7063804726825182 1101 19 0.7231932520360189 1101 20 0.5876607830933551 1101 21 0.8026119421326143 1101 22 0.8260553910031163 1101 23 0.6989537876534513 1101 24 0.5741123504704007 1101 25 0.829559522009987 1102 1 1.3522204011338417 1102 2 3.956430996206361 1102 3 2.2319337863667776 1102 4 1.3678833753954933 1102 5 1.1006980662654016 1102 6 1.0420303492193952 1102 7 0.7456411472917495 1102 8 0.8771996854432516 1102 9 0.9278523783329149 1102 10 0.9104623589991121 1102 11 0.7339904411235239 1102 12 1.1383289747251126 1102 13 1.123481636951297 1102 14 1.3011172266771638 1102 15 0.9457902791232229 1102 16 1.2245454394345359 1102 17 1.5963377982989257 1102 18 1.235156920430235 1102 19 1.4457880597061958 1102 20 1.4288946326698095 1102 21 1.4930475277966195 1102 22 1.3513103899261694 1102 23 1.3256816875009108 1102 24 1.1973645345948185 1102 25 1.3681376061175925 1103 1 0.6775553473106761 1103 2 3.320457728813089 1103 3 1.8097678856636286 1103 4 0.8069704182003008 1103 5 0.4935790628271377 1103 6 0.41308172843373825 1103 7 0.49634848065525716 1103 8 0.28817854155868616 1103 9 0.1833673842233594 1103 10 0.32568041516611146 1103 11 0.49690381798717453 1103 12 0.2806322414189353 1103 13 0.4930285184443264 1103 14 0.5219862695450672 1103 15 0.1929091728442387 1103 16 0.2695363671898434 1103 17 0.32854680416468085 1103 18 0.4878192779521058 1103 19 0.4391871959571887 1103 20 0.5540189217363434 1103 21 0.4464094699015675 1103 22 0.5314139904347144 1103 23 0.7835399794502678 1103 24 0.614957969114883 1103 25 0.7814986979138965 1104 1 1.1430451524051968 1104 2 3.700494090316816 1104 3 1.891960523436584 1104 4 1.2431677086817956 1104 5 0.8379264129336832 1104 6 0.769490932195005 1104 7 0.5079176945821047 1104 8 0.6341758437696967 1104 9 0.6956657831442428 1104 10 0.8103206122220072 1104 11 0.4875593025139864 1104 12 0.7114965113542269 1104 13 0.5618768753344412 1104 14 0.8705093278212175 1104 15 0.9052466701562238 1104 16 0.8240375694653679 1104 17 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0.21312644036252237 2314 5 -0.27410532324989134 2314 6 -0.27662899974517074 2314 7 -0.35145228201058387 2314 8 -0.4728886532550334 2314 9 -0.5795431435974407 2314 10 -0.7041909833296671 2314 11 -0.5089390006906527 2314 12 -0.6146125159731071 2314 13 -0.5561110355861487 2314 14 -1.4106087710159372 2314 15 -0.956967906302971 2314 16 -0.92256272947143 2314 17 -0.7982641433718172 2314 18 -0.6943185277427446 2314 19 -0.6344943075730711 2314 20 -0.6532672813814884 2314 21 -0.584934867774777 2314 22 -0.7021874466165698 2314 23 -0.5671989850207098 2314 24 -0.5203394110684698 2314 25 -0.4503812714566493 2315 1 -4.523385337545715 2315 2 -13.99332826774561 2315 3 -7.116671503978438 2315 4 -3.1903786467873125 2315 5 -1.9711825988288467 2315 6 -1.376762656764896 2315 7 -1.1576005049177596 2315 8 -1.2852601106620867 2315 9 -1.2622345595435434 2315 10 -1.4153022459793476 2315 11 -1.463992054344402 2315 12 -1.2937688401322944 2315 13 -1.3136394717990214 2315 14 -1.5358303961761877 2315 15 -1.6011347320851357 2315 16 -1.6248649034437719 2315 17 -1.3636006044612543 2315 18 -1.5328417540595396 2315 19 -1.715876430264757 2315 20 -1.415904688980845 2315 21 -1.528167458266033 2315 22 -1.5592576301903947 2315 23 -1.790616240414728 2315 24 -1.9338687746973946 2315 25 -1.7945180926017343 2316 1 -1.25385793181459 2316 2 -1.1680434218123956 2316 3 -0.6864580997735068 2316 4 -0.6605204776953997 2316 5 -0.9451017631053489 2316 6 -1.0287769714242643 2316 7 -1.0248978235504964 2316 8 -1.2390782302842211 2316 9 -1.1992540053767655 2316 10 -1.4735173868892772 2316 11 -1.5927292593019047 2316 12 -1.3695220701404551 2316 13 -1.3066071322468815 2316 14 -1.445738824458168 2316 15 -2.2741105497837424 2316 16 -1.972303263573238 2316 17 -1.7971611128421756 2316 18 -2.0079410883226245 2316 19 -1.7925374833395153 2316 20 -1.970120145655315 2316 21 -2.203836484395545 2316 22 -2.011244315452082 2316 23 -1.9752174561082256 2316 24 -1.9567695983165834 2316 25 -2.004298899380146 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 28224.0 3 1.0 4 246.0 5 1327.0 6 1563.0 7 1388.0 8 1267.0 9 1222.0 10 1241.0 11 1255.0 12 1183.0 13 1166.0 14 1186.0 15 1236.0 16 1266.0 17 1443.0 18 1589.0 19 2019.0 20 2603.0 21 3326.0 22 3952.0 23 4986.0 24 5829.0 25 6707.0 26 7721.0 27 8911.0 28 10307.0 29 12529.0 30 15930.0 31 20698.0 32 27003.0 33 36497.0 34 50134.0 35 73726.0 36 115250.0 37 156861.0 38 377733.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.45936181501225 19.843258128900228 13.21947399004573 24.47790606604179 2 16.087512233209083 21.998171034969598 39.939462327063865 21.974854404757448 3 18.068669311033073 25.803895808595033 30.59781208155428 25.529622798817613 4 11.93602991334226 17.75710568201915 39.54917763573432 30.757686768904275 5 12.606174894368296 38.335743585545764 36.292334791611715 12.765746728474225 6 30.02135380927501 38.27778951187395 18.839254223516676 12.861602455334358 7 26.418129910815797 32.503979181930724 23.42457239584649 17.653318511406987 8 24.08205957403805 37.162881180364316 21.718602359718048 17.036456885879588 9 26.057856042040374 15.33281119729163 21.37116293170966 37.23816982895834 10 14.687855284100957 29.33063843763422 35.40516914681287 20.57633713145196 11 32.70720800384023 23.050857734771736 25.771051767262072 18.47088249412597 12 22.489174098683712 25.467168590990624 32.709734468558146 19.333922841767514 13 28.23496121876658 22.80872135620626 26.545665849776405 22.41065157525075 14 20.313584800788256 23.188398474015308 28.890629342361233 27.607387382835196 15 22.28180187463682 31.6650918369925 24.411712690432278 21.641393597938404 16 21.11841540132892 28.911851645991764 28.826558197114778 21.14317475556454 17 20.796547821165817 29.18071388147789 28.74231950844854 21.280418788907753 18 20.6042293019378 27.802329400469922 31.02114650968899 20.572294787903285 19 22.4512668250598 27.424885373832392 29.060626303029448 21.063221498078363 20 22.648028692670493 27.71229716196018 29.567133997156198 20.072540148213125 21 22.254145701093158 27.199780903651167 28.580855951224788 21.965217444030888 22 22.281332239169192 28.565465915843685 28.736260626664993 20.41694121832213 23 21.397322542927334 28.239521950519492 29.19766230327603 21.16549320327714 24 22.076379912703473 27.24292345333658 29.83604867503924 20.84464795892071 25 21.771566011803703 27.84440941062333 29.506326299620017 20.87769827795295 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 281.0 1 281.0 2 385.0 3 489.0 4 489.0 5 489.0 6 1235.5 7 1982.0 8 1982.0 9 1982.0 10 2723.0 11 3464.0 12 3464.0 13 3464.0 14 5185.5 15 6907.0 16 6907.0 17 6907.0 18 11618.5 19 16330.0 20 16330.0 21 16330.0 22 24430.5 23 32531.0 24 32531.0 25 32531.0 26 45056.0 27 57581.0 28 57581.0 29 57581.0 30 71679.5 31 85778.0 32 85778.0 33 85778.0 34 94958.0 35 104138.0 36 104138.0 37 104138.0 38 109605.5 39 115073.0 40 115073.0 41 115073.0 42 118470.0 43 121867.0 44 121867.0 45 121867.0 46 122877.0 47 123887.0 48 123887.0 49 123887.0 50 117667.5 51 111448.0 52 111448.0 53 111448.0 54 97956.0 55 84464.0 56 84464.0 57 84464.0 58 70982.0 59 57500.0 60 57500.0 61 57500.0 62 46487.0 63 35474.0 64 35474.0 65 35474.0 66 26762.0 67 18050.0 68 18050.0 69 18050.0 70 13016.0 71 7982.0 72 7982.0 73 7982.0 74 5470.5 75 2959.0 76 2959.0 77 2959.0 78 1948.5 79 938.0 80 938.0 81 938.0 82 619.0 83 300.0 84 300.0 85 300.0 86 182.5 87 65.0 88 65.0 89 65.0 90 44.0 91 23.0 92 23.0 93 23.0 94 15.5 95 8.0 96 8.0 97 8.0 98 7.0 99 6.0 100 6.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.0 2 5.514767186276243 3 0.0 4 0.0 5 2.0211717743361715E-4 6 6.063515323008514E-4 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 5.052929435840428E-4 18 0.0 19 4.042343548672343E-4 20 4.042343548672343E-4 21 6.063515323008514E-4 22 0.0033349334276546825 23 4.042343548672343E-4 24 0.0024254061292034055 25 5.052929435840428E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 989525.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 79.81575663811795 #Duplication Level Percentage of deduplicated Percentage of total 1 88.81340426248634 70.88709060817398 2 8.446274721251058 13.482916153001238 3 1.5877120242711324 3.8017330962191496 4 0.4562231341811841 1.4565517860193928 5 0.19846925594446072 0.7920486916305709 6 0.11883637957326566 0.569100933106487 7 0.07732804910093062 0.43203977238382196 8 0.04842055415977258 0.30917785336793746 9 0.03761945118685995 0.2702362464710974 >10 0.18350862229697337 2.72196626898654 >50 0.016362160966537094 0.9290593459207149 >100 0.01379733635049121 2.088081878816004 >500 8.942711010300468E-4 0.4767978561163509 >1k 0.0011497771298957743 1.7831995097867286 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3983 0.4025163588590485 No Hit GGTATCAACGCAGAGTACTTTTTTT 2822 0.2851873373588338 No Hit TATCAACGCAGAGTACTTTTTTTTT 2779 0.28084181804401104 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1494 0.150981531542912 No Hit GTCCTACAGTGGACATTTCTAAATT 1469 0.1484550668249918 No Hit CTGTAGGACGTGGAATATGGCAAGA 1314 0.13279098557388647 No Hit CTTTAGGACGTGAAATATGGCGAGG 1262 0.1275359389606124 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1216 0.12288724387963923 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 1149 0.11611631843561304 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 1.0105858871680857E-4 0.0 0.0 0.0 0.0 7 1.0105858871680857E-4 0.0 0.0 0.0 0.0 8 1.0105858871680857E-4 0.0 0.0 0.0 0.0 9 1.0105858871680857E-4 0.0 0.0 0.0 0.0 10 1.0105858871680857E-4 0.0 0.0 0.0 0.0 11 1.0105858871680857E-4 0.0 0.0 0.0 0.0 12 1.0105858871680857E-4 0.0 0.0 1.0105858871680857E-4 0.0 13 1.0105858871680857E-4 0.0 0.0 1.0105858871680857E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCAACGA 25 0.0062352596 18.890493 19 GATAGCG 25 0.0062370794 18.889542 7 CTTCGTG 40 0.005485401 14.167155 9 TAGGACC 285 0.0 13.918609 4 GGTATCA 755 0.0 13.504298 1 CTAGGAC 115 5.7934813E-9 12.319266 3 GGATAAC 65 5.228709E-4 12.302559 1 GTCTTAG 115 3.4804543E-8 12.168836 1 CTTAGAC 80 3.0399277E-5 11.805963 3 AGGCGAA 65 8.4207713E-4 11.624332 9 GTCCTAT 125 1.10740075E-7 11.195328 1 TGGTATC 250 0.0 11.195328 2 GACGTGG 405 0.0 11.193802 7 TAGACTG 135 5.167749E-9 11.193801 5 AATCCCG 70 0.001564187 10.794568 19 CTCCGGG 65 0.007294796 10.764739 2 TAGCACC 80 3.9765862E-4 10.625366 4 GTATAAG 160 2.6630005E-9 10.620568 1 ACGAAAT 90 1.0084834E-4 10.494189 12 GTAGGAC 950 0.0 10.438956 3 >>END_MODULE