FastQCFastQC Report
Thu 26 May 2016
SRR1511867_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511867_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences989525
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT43080.4353604001920113No Hit
TATCAACGCAGAGTACTTTTTTTTT31670.32005255046613273No Hit
GGTATCAACGCAGAGTACTTTTTTT30810.3113615118364872No Hit
ACGCAGAGTACTTTTTTTTTTTTTT15180.1534069376721154No Hit
GTCCTACAGTGGACATTTCTAAATT15120.15280058613981456No Hit
CTGTAGGACGTGGAATATGGCAAGA15090.15249741037366413No Hit
CTTTAGGACGTGAAATATGGCGAGG14660.14815189105884138No Hit
GTCCTAAAGTGTGTATTTCTCATTT13900.1404714383163639No Hit
GTACTTTTTTTTTTTTTTTTTTTTT11980.12106818928273666No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7200.014.83801651
GTCTTCG400.005136758614.3080871
ATAGGAC1207.366907E-1012.6687893
GTAGAAC2400.011.876993
TTATACC658.007632E-411.6942674
ATTATAC1406.7666406E-1011.5376483
GTAAGAC855.3090203E-511.1783453
GGCGAGG4100.010.88656619
GGCGTGC700.001494778710.8534778
TGGCGAG8850.010.73083718
GTCTAGG909.121926E-510.5985831
TGTAGAA3050.010.5919392
TAGGACC3250.010.524844
CTGTGCA2400.010.288199
TAGGACA2900.010.1568764
TATGGCG9650.010.13647216
GTAATAC750.002642438710.1350333
TCAGTAC750.002642438710.1350333
GTCTTAC856.346505E-410.0998271
GGTCTAC856.346505E-410.0998271
Produced by FastQC (version 0.11.5)