FastQCFastQC Report
Thu 26 May 2016
SRR1511866_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511866_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1884169
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT67570.358619635499788No Hit
TATCAACGCAGAGTACTTTTTTTTT43080.22864191057171626No Hit
GGTATCAACGCAGAGTACTTTTTTT42130.22359990000896945No Hit
GTCCTACAGTGGACATTTCTAAATT32110.17041995702084048No Hit
CTGTAGGACGTGGAATATGGCAAGA30670.16277733048362433No Hit
GTCCTAAAGTGTGTATTTCTCATTT30010.15927445998740028No Hit
CTTTAGGACGTGAAATATGGCGAGG28500.1512603168824028No Hit
ACGCAGAGTACTTTTTTTTTTTTTT19250.10216705613986855No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACGTA402.8869996E-416.5284066
CGGTAGG755.448928E-714.6600141
GGTATCA11000.013.3575751
GTCAACG655.234426E-412.302112
GTCGAGG951.1152533E-611.9309119
TAGGACC5700.011.9302774
GGCGAGG7850.011.55094519
TCCAACG907.958062E-611.5442618
GTCCTAA9800.011.42338851
GTATCAA23650.010.8196151
TGTCGAG1159.498872E-710.677299518
CGACTAA901.0090147E-410.49478217
ATGTCGA1106.477374E-610.30396717
TGGCGAG17600.010.25030118
AATGTCC6200.010.206488
CTACACT2900.010.09616854
GGGCTAT1501.2629607E-79.9954641
TGGTATC3500.09.9954642
TTAGACT1804.3837645E-109.9695144
AAATGTC6200.09.9018097
Produced by FastQC (version 0.11.5)