FastQCFastQC Report
Thu 26 May 2016
SRR1511866_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511866_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1884169
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT84630.44916353044764035No Hit
TATCAACGCAGAGTACTTTTTTTTT58720.3116493265731471No Hit
GGTATCAACGCAGAGTACTTTTTTT57690.30618272564722165No Hit
GTCCTACAGTGGACATTTCTAAATT33510.1778502883764673No Hit
CTGTAGGACGTGGAATATGGCAAGA32470.17233061365514452No Hit
GTCCTAAAGTGTGTATTTCTCATTT31230.16574946302587507No Hit
CTTTAGGACGTGAAATATGGCGAGG30940.16421032295935237No Hit
ACGCAGAGTACTTTTTTTTTTTTTT28010.14865970090793343No Hit
GTACTTTTTTTTTTTTTTTTTTTTT20900.11092423238042873No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14550.013.82980251
GGCGAGG8600.012.25929819
TAGGACC6050.011.6182434
GTACTAG1354.452886E-911.3027151
TCCAACG1303.276E-810.95945618
GCGAACC700.001494684610.85450511
TGGTATC3950.010.8213032
TTAGACA1909.094947E-1210.4985634
GTATAAA3300.010.4036341
GCGTGCA1201.5286296E-610.28916559
GTGGTAT4700.010.1453891
TGTAGGA19550.09.9602742
TTATACT3550.09.9000534
TGGCGAG19300.09.842689518
AGGCGTG1451.6228296E-79.8251987
GTATCAA29800.09.8246851
GGACTGT2350.09.6997696
GACGTGA11300.09.6657927
ATAGGAC2950.09.6596953
GGACGTG19800.09.6415976
Produced by FastQC (version 0.11.5)