##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511866_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1884169 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.78093737875955 31.0 31.0 34.0 27.0 34.0 2 30.979246023047825 31.0 31.0 34.0 27.0 34.0 3 31.083506309678167 31.0 31.0 34.0 27.0 34.0 4 34.66881420934109 37.0 35.0 37.0 32.0 37.0 5 34.29397362975402 37.0 35.0 37.0 30.0 37.0 6 34.2346774625843 37.0 35.0 37.0 30.0 37.0 7 34.069022470914234 37.0 35.0 37.0 30.0 37.0 8 33.980538900703706 37.0 35.0 37.0 30.0 37.0 9 35.46752228701353 39.0 35.0 39.0 30.0 39.0 10 35.273349152862615 39.0 35.0 39.0 29.0 39.0 11 35.25391989784356 39.0 35.0 39.0 29.0 39.0 12 35.085556550394365 38.0 35.0 39.0 29.0 39.0 13 35.06660761322365 38.0 35.0 39.0 29.0 39.0 14 36.063768695907854 39.0 36.0 41.0 28.0 41.0 15 35.99354357278992 39.0 36.0 41.0 27.0 41.0 16 35.89412998515526 39.0 36.0 41.0 27.0 41.0 17 35.84247432157094 39.0 36.0 41.0 27.0 41.0 18 35.77606095843844 39.0 36.0 41.0 27.0 41.0 19 35.799821565899876 39.0 36.0 41.0 27.0 41.0 20 35.65795796449257 39.0 36.0 41.0 27.0 41.0 21 35.554631776661225 39.0 36.0 41.0 27.0 41.0 22 35.62620497418225 39.0 36.0 41.0 27.0 41.0 23 35.518147257491236 39.0 36.0 41.0 27.0 41.0 24 35.390144408489896 39.0 36.0 41.0 27.0 41.0 25 34.94349073782659 39.0 35.0 40.0 25.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2971758383922314 1101 2 0.28792590673997864 1101 3 0.27134437705613124 1101 4 0.2572718453770264 1101 5 0.32373703995757097 1101 6 0.38645470040084007 1101 7 0.42342630578837515 1101 8 0.47587654443105976 1101 9 0.3688577165915916 1101 10 0.4482085474908999 1101 11 0.2706211152050173 1101 12 0.49321062706277985 1101 13 0.45399467287926853 1101 14 0.3007648988619067 1101 15 0.22680634439591074 1101 16 0.33251757588229225 1101 17 0.4198104374794127 1101 18 0.1906377171214615 1101 19 0.4143815078420161 1101 20 0.2691892911511786 1101 21 0.3347329306191469 1101 22 0.053416354795672305 1101 23 -0.01757450967490115 1101 24 -0.09558621564520564 1101 25 -0.06379950695722414 1102 1 0.627195090189911 1102 2 0.6554508559712993 1102 3 0.6410716206680505 1102 4 0.5957628867644686 1102 5 0.5609136236657974 1102 6 0.691391691599506 1102 7 0.7247465411467715 1102 8 0.8245659028732817 1102 9 0.8115513419568927 1102 10 0.9013027086824437 1102 11 0.8818796007255116 1102 12 0.8537385214838977 1102 13 1.001086681350344 1102 14 1.1892656767087146 1102 15 1.3466047390570495 1102 16 1.307834403123266 1102 17 1.385224251268376 1102 18 1.344048652056948 1102 19 1.438492619032381 1102 20 1.4653201006610956 1102 21 1.6351633320537218 1102 22 1.2563844413210532 1102 23 1.4420038481374817 1102 24 1.4121202097760133 1102 25 1.5672165014938741 1103 1 0.4579412617645673 1103 2 0.4216700753903808 1103 3 0.3902580438322971 1103 4 0.3700845973101963 1103 5 0.6224655227664897 1103 6 0.5362398616605688 1103 7 0.5457637540517553 1103 8 0.4504756234611875 1103 9 0.7647532633910643 1103 10 0.7313356497103811 1103 11 0.6612742021294693 1103 12 0.7521805295623736 1103 13 0.6370982714324995 1103 14 0.9650560452489003 1103 15 0.8628155534023492 1103 16 0.7047765343618053 1103 17 0.5745909066596653 1103 18 0.791770808448959 1103 19 0.8301007875290622 1103 20 0.8269070609267146 1103 21 0.7002409023081313 1103 22 0.8023639686197015 1103 23 0.8193830843804335 1103 24 0.9352468560555138 1103 25 0.9227113270560068 1104 1 0.6069664758152342 1104 2 0.4666754392065613 1104 3 0.5599462117376852 1104 4 0.6166331733396149 1104 5 0.7346161875735717 1104 6 0.605574139490777 1104 7 0.4668404409875748 1104 8 0.5426045565012032 1104 9 0.8373315061462918 1104 10 0.7572778231965103 1104 11 0.7173890348669474 1104 12 0.7966934558820711 1104 13 0.897004872892893 1104 14 0.9770309006529914 1104 15 0.8635463095802862 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-0.16403425874992195 2313 20 -0.2790004687662204 2313 21 -0.32454216202327757 2313 22 -0.3137591216421569 2313 23 -0.15877220806945047 2313 24 -0.25956935232032663 2313 25 0.07983216504093349 2314 1 -0.44131094388932013 2314 2 -0.5209401028635305 2314 3 -0.5628183831520062 2314 4 -0.5751363336206552 2314 5 -0.5525060824586703 2314 6 -0.6219048041688353 2314 7 -0.5431316043490497 2314 8 -0.5384505449022043 2314 9 -0.6115465645431328 2314 10 -0.8032038849547405 2314 11 -0.8659620658963192 2314 12 -0.824994510190308 2314 13 -0.7307306445006958 2314 14 -0.9285605482658639 2314 15 -0.7545816314025942 2314 16 -0.7500701599642028 2314 17 -0.9703694450961109 2314 18 -0.8997937893374299 2314 19 -0.793271441206187 2314 20 -0.7384617578245454 2314 21 -0.6522686735848993 2314 22 -0.40142435127125964 2314 23 -0.7001125721981438 2314 24 -0.6990106891215504 2314 25 -0.7568514847969539 2315 1 -0.49712014552148887 2315 2 -0.4865429375539172 2315 3 -0.6158828159772938 2315 4 -0.614884964734344 2315 5 -0.700871135269594 2315 6 -0.5662832649665219 2315 7 -0.6348250725853362 2315 8 -0.6378840595190951 2315 9 -0.7747869514734305 2315 10 -0.8427864595104282 2315 11 -0.8514112313952396 2315 12 -0.963905862326925 2315 13 -0.8760626444430741 2315 14 -0.9793678876776468 2315 15 -1.2609463097117413 2315 16 -1.1346067345898518 2315 17 -1.2416280797284713 2315 18 -1.225982369502411 2315 19 -1.2143830530248039 2315 20 -1.2336891236160668 2315 21 -1.1425345081168459 2315 22 -1.253398407763413 2315 23 -1.2553266863882655 2315 24 -1.1324849750474897 2315 25 -1.0419329389338614 2316 1 -1.6044001999912503 2316 2 -1.1758749794916135 2316 3 -1.0707361018757062 2316 4 -1.10391061426364 2316 5 -1.1320590936333872 2316 6 -1.1632221045696554 2316 7 -1.190465004347388 2316 8 -1.1874068209953066 2316 9 -1.4164893576399677 2316 10 -1.5400446306027646 2316 11 -1.2185111688354127 2316 12 -1.489723725293267 2316 13 -1.3540033960152371 2316 14 -1.7509095728735815 2316 15 -1.604441218246258 2316 16 -1.6652151980049155 2316 17 -1.587183191419122 2316 18 -1.6723523920390235 2316 19 -1.7387732203283761 2316 20 -1.5985065067058244 2316 21 -1.83519993363673 2316 22 -1.8051434249610878 2316 23 -1.7384016149720765 2316 24 -1.5059325994071244 2316 25 -1.5622246838003093 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 31496.0 3 2.0 4 555.0 5 4992.0 6 6345.0 7 4930.0 8 4090.0 9 3619.0 10 3435.0 11 3234.0 12 3000.0 13 3011.0 14 2941.0 15 2983.0 16 3041.0 17 3162.0 18 3465.0 19 3930.0 20 4847.0 21 5990.0 22 7950.0 23 9825.0 24 11952.0 25 14256.0 26 16607.0 27 18892.0 28 22005.0 29 26781.0 30 33976.0 31 43505.0 32 55799.0 33 73324.0 34 94919.0 35 128430.0 36 189050.0 37 339681.0 38 698149.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.55112889448749 20.417774353252245 13.897676669016587 24.133420083243678 2 14.852277051580828 21.52190169777764 40.93327084778488 22.692550402856643 3 18.373086490649193 25.961418535173863 30.800581051912012 24.864913922264932 4 12.501957096205278 18.1499642547988 38.900597557862376 30.447481091133543 5 12.630507707964266 38.5277705962889 36.14013051965254 12.701591176094295 6 30.409851770196834 38.51857237859237 18.379561493687667 12.692014357523131 7 26.853642109598447 32.343913948271094 23.090285425564268 17.71215851656619 8 24.352061837340493 37.52327949350616 21.149642096860738 16.97501657229261 9 25.996553387726895 15.291940372652347 21.02019510988664 37.69131112973412 10 14.645713839894404 29.63502743119115 35.07265006482964 20.646608664084802 11 33.24415166580068 22.848215844757025 25.5505212112077 18.357111278234594 12 22.739998375941862 25.432007426085452 32.57319274438758 19.254801453585106 13 28.503281818138394 22.638627426732953 26.360958066924994 22.497132688203656 14 20.31717961605355 23.07234648271997 28.661547876013245 27.94892602521324 15 22.345341633367283 31.800225988220802 24.199793118345543 21.654639260066375 16 21.213140873393918 28.884913067684554 28.691724247372978 21.210221811548553 17 20.88140713492261 29.261387911593918 28.615320600222166 21.241884353261305 18 20.632183542019607 27.891939572267443 31.04548002088986 20.430396864823095 19 22.776937737538404 27.276003373370433 28.859513132845304 21.087545756245856 20 22.7372916123766 27.743901953593337 29.419866264650356 20.098940169379713 21 22.25644302607675 27.11603895404287 28.520371580256338 22.107146439624046 22 22.399476904672564 28.34634260514848 28.749172712214243 20.505007777964714 23 21.490959675060996 28.20527245698236 29.110764480256286 21.193003387700358 24 22.251850640923912 27.274840677955307 29.75732945097255 20.71597923014824 25 21.924743441136883 27.902978927569094 29.29744056793237 20.874837063361653 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 276.0 1 276.0 2 553.5 3 831.0 4 831.0 5 831.0 6 2067.0 7 3303.0 8 3303.0 9 3303.0 10 4746.5 11 6190.0 12 6190.0 13 6190.0 14 9040.0 15 11890.0 16 11890.0 17 11890.0 18 20410.5 19 28931.0 20 28931.0 21 28931.0 22 44186.0 23 59441.0 24 59441.0 25 59441.0 26 83380.5 27 107320.0 28 107320.0 29 107320.0 30 135404.5 31 163489.0 32 163489.0 33 163489.0 34 181001.5 35 198514.0 36 198514.0 37 198514.0 38 209005.0 39 219496.0 40 219496.0 41 219496.0 42 226553.5 43 233611.0 44 233611.0 45 233611.0 46 235776.5 47 237942.0 48 237942.0 49 237942.0 50 226486.0 51 215030.0 52 215030.0 53 215030.0 54 187928.5 55 160827.0 56 160827.0 57 160827.0 58 135852.0 59 110877.0 60 110877.0 61 110877.0 62 89155.0 63 67433.0 64 67433.0 65 67433.0 66 51262.5 67 35092.0 68 35092.0 69 35092.0 70 25207.0 71 15322.0 72 15322.0 73 15322.0 74 10547.0 75 5772.0 76 5772.0 77 5772.0 78 3827.5 79 1883.0 80 1883.0 81 1883.0 82 1203.0 83 523.0 84 523.0 85 523.0 86 333.5 87 144.0 88 144.0 89 144.0 90 86.0 91 28.0 92 28.0 93 28.0 94 16.0 95 4.0 96 4.0 97 4.0 98 2.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3985842034339807 2 0.0 3 0.0 4 0.0 5 0.024838536245952458 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 1.0614759079466862E-4 17 0.0 18 5.307379539733431E-5 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.004882789176554757 25 5.307379539733431E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1884169.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.80663848695767 #Duplication Level Percentage of deduplicated Percentage of total 1 83.69087388629703 60.095603258108966 2 11.404670047338202 16.37862038304497 3 2.7493172347021915 5.922576862746673 4 0.931199479770306 2.6746521761243764 5 0.4071044537981567 1.4616401170157298 6 0.21773331248471842 0.9380818353694778 7 0.11775160841794662 0.5918743023847706 8 0.08465156875218795 0.48628356757937613 9 0.058589491836671774 0.3786403013505403 >10 0.29630604901347346 3.74810044060533 >50 0.021967588048212777 1.0939753980773732 >100 0.0160019032658849 2.2865604755026703 >500 0.0024327195592669204 1.2321144529367438 >1k 0.0011795003923718368 1.6470717134871415 >5k 2.211563235697194E-4 1.0642047156659327 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 8463 0.44916353044764035 No Hit TATCAACGCAGAGTACTTTTTTTTT 5872 0.3116493265731471 No Hit GGTATCAACGCAGAGTACTTTTTTT 5769 0.30618272564722165 No Hit GTCCTACAGTGGACATTTCTAAATT 3351 0.1778502883764673 No Hit CTGTAGGACGTGGAATATGGCAAGA 3247 0.17233061365514452 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3123 0.16574946302587507 No Hit CTTTAGGACGTGAAATATGGCGAGG 3094 0.16421032295935237 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2801 0.14865970090793343 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 2090 0.11092423238042873 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1455 0.0 13.8298025 1 GGCGAGG 860 0.0 12.259298 19 TAGGACC 605 0.0 11.618243 4 GTACTAG 135 4.452886E-9 11.302715 1 TCCAACG 130 3.276E-8 10.959456 18 GCGAACC 70 0.0014946846 10.854505 11 TGGTATC 395 0.0 10.821303 2 TTAGACA 190 9.094947E-12 10.498563 4 GTATAAA 330 0.0 10.403634 1 GCGTGCA 120 1.5286296E-6 10.2891655 9 GTGGTAT 470 0.0 10.145389 1 TGTAGGA 1955 0.0 9.960274 2 TTATACT 355 0.0 9.900053 4 TGGCGAG 1930 0.0 9.8426895 18 AGGCGTG 145 1.6228296E-7 9.825198 7 GTATCAA 2980 0.0 9.824685 1 GGACTGT 235 0.0 9.699769 6 GACGTGA 1130 0.0 9.665792 7 ATAGGAC 295 0.0 9.659695 3 GGACGTG 1980 0.0 9.641597 6 >>END_MODULE